Abstract
Transforming growth factor beta1 (TGFbeta1) regulates cellular functions including cell growth, differentiation, angiogenesis, migration, and metastasis. The TGFbeta1 signal transduction pathways are mostly undefined in mouse dental papilla-derived MDPC-23 cells. In this study, we investigated TGFbeta1-induced migration focusing on heat shock protein 27 (Hsp27) activation. Cellular responses mediated by TGFbeta1 in MDPC-23 cells were measured by Western blot and MTT assays. Cell migration was determined by counting migrated cells using the chemotaxis cell migration assay. TGFbeta1 induced cell migration and increased the phosphorylation of Hsp27 and p38 MAPK in MDPC-23 cells. However, TGFbeta1 did not affect Akt/NF-kappaB signaling to regulate the migration of MDPC-23 cells. Inhibiting p38 MAPK with SB203580 blocked TGFbeta1-induced Hsp27 activation and cell migration. Hsp27 phosphorylation followed by p38 MAPK activation was required for TGFbeta1-induced migration, and Hsp27 itself contributed to MDPC-23 cell migration.
References
Mar 1, 1992·Journal of Cellular Biochemistry·A R ShakooriG S Stein
Oct 23, 1987·Cell·R A Ignotz, J Massagué
Oct 6, 1993·Journal of the National Cancer Institute·D R CioccaS A Fuqua
Dec 23, 1998·Connective Tissue Research·C T HanksW T Butler
Feb 26, 2000·Journal of Dental Research·H PalosaariL Tjäderhane
Nov 10, 2000·Archives of Histology and Cytology·H OhshimaT Maeda
Jan 5, 2002·Archives of Histology and Cytology·Y OtsukaH Ohshima
Mar 23, 2002·Biochimica Et Biophysica Acta·Daijiro HatakeyamaToshihiko Uematsu
Jan 15, 2004·The Biochemical Journal·Hwang-Phill KimYung-Jue Bang
Sep 9, 2004·Archives of Oral Biology·Wen-Xi HeAnthony J Smith
Sep 27, 2005·Archives of Oral Biology·Wen-Xi HeAnthony J Smith
Nov 2, 2005·Archives of Oral Biology·Naohiro NakasoneHayato Ohshima
Dec 23, 2006·Annals of the New York Academy of Sciences·Naing Naing MonMichinari Hamaguchi
Jul 12, 2007·Acta Biochimica Et Biophysica Sinica·Kuansong WangJifang Wen
Oct 12, 2007·Journal of Molecular Histology·Yun-Chan HwangHo-Hyun Son
Dec 29, 2007·Molecular Carcinogenesis·Ying-Ying WeiChih-Hsin Tang
Jan 3, 2008·Cancer Science·Kun GuoPei Chen
Jan 15, 2008·Biochemical Pharmacology·Ying-Yi YehChih-Hsin Tang
Mar 25, 2008·Journal of Endodontics·Tseng-Fang TaiMei-Chi Chang
May 13, 2008·European Journal of Cell Biology·Joong-Won LeeJae-Seon Lee
Dec 3, 2008·International Journal of Cancer. Journal International Du Cancer·Kiwon S YooJohn J Krolewski
Mar 27, 2009·World Journal of Gastroenterology : WJG·Julia BrenmoehlGerhard Rogler
Jul 18, 2009·Molecular and Cellular Biochemistry·Hai-Feng ChenChang-Sheng Xu
Feb 23, 2010·Journal of Endodontics·Ivarne L TersariolLeo Tjäderhane
Citations
Jan 12, 2011·Cell Stress & Chaperones·Min Jia, Serhiy Souchelnytskyi
May 15, 2013·Molecular Pain·Elias UtrerasAshok B Kulkarni
Jan 1, 2016·Proteome Science·Emanuela MonariCarlo Bertoldi
Dec 17, 2014·PloS One·Maja SabolSonja Levanat
Aug 23, 2011·Archives of Oral Biology·A J SmithP R Cooper
May 19, 2018·International Endodontic Journal·Y ChaeJ Kim
Mar 6, 2019·BioMed Research International·Jun KangFang Huang
Apr 7, 2011·Biological & Pharmaceutical Bulletin·Seong-Min KwonJung-Hoon Yoon
Nov 5, 2015·Oncology Reports·Hyun-Woo KimSang-Gun Ahn
Aug 14, 2012·Archives of Toxicology·Daniel R CioccaStuart K Calderwood
Feb 22, 2012·Journal of Endodontics·Yusuke YamanakaTakashi Okiji
Apr 9, 2011·Steroids·Changxue Lu, Sheue-Yann Cheng