Transforming growth factor beta1-induced heat shock protein 27 activation promotes migration of mouse dental papilla-derived MDPC-23 cells

Journal of Endodontics
Seong-Min KwonSang-Gun Ahn

Abstract

Transforming growth factor beta1 (TGFbeta1) regulates cellular functions including cell growth, differentiation, angiogenesis, migration, and metastasis. The TGFbeta1 signal transduction pathways are mostly undefined in mouse dental papilla-derived MDPC-23 cells. In this study, we investigated TGFbeta1-induced migration focusing on heat shock protein 27 (Hsp27) activation. Cellular responses mediated by TGFbeta1 in MDPC-23 cells were measured by Western blot and MTT assays. Cell migration was determined by counting migrated cells using the chemotaxis cell migration assay. TGFbeta1 induced cell migration and increased the phosphorylation of Hsp27 and p38 MAPK in MDPC-23 cells. However, TGFbeta1 did not affect Akt/NF-kappaB signaling to regulate the migration of MDPC-23 cells. Inhibiting p38 MAPK with SB203580 blocked TGFbeta1-induced Hsp27 activation and cell migration. Hsp27 phosphorylation followed by p38 MAPK activation was required for TGFbeta1-induced migration, and Hsp27 itself contributed to MDPC-23 cell migration.

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Citations

Jan 12, 2011·Cell Stress & Chaperones·Min Jia, Serhiy Souchelnytskyi
May 15, 2013·Molecular Pain·Elias UtrerasAshok B Kulkarni
Jan 1, 2016·Proteome Science·Emanuela MonariCarlo Bertoldi
Aug 23, 2011·Archives of Oral Biology·A J SmithP R Cooper
Mar 6, 2019·BioMed Research International·Jun KangFang Huang
Aug 14, 2012·Archives of Toxicology·Daniel R CioccaStuart K Calderwood
Feb 22, 2012·Journal of Endodontics·Yusuke YamanakaTakashi Okiji

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