Transforming growth factor-beta1 inhibits type I inositol 1,4,5-trisphosphate receptor expression and enhances its phosphorylation in mesangial cells.

The Journal of Biological Chemistry
K SharmaS K Joseph

Abstract

A potentially important cross-talk characteristic of transforming growth factor-beta (TGF-beta) is to inhibit platelet-derived growth factor-induced intracellular calcium rise (Baffy, G., Sharma, K., Shi, W., Ziyadeh, F. N., and Williamson, J. R. (1995) Biochem. Biophys. Res. Commun. 210, 378-383) in murine mesangial cells. The present study examined the possible basis for this effect by evaluating the regulation of the type I inositol 1,4,5-trisphosphate receptor (IP3R) by TGF-beta. TGF-beta1 down-regulates IP3R protein expression by >90% with maximal and half-maximal effects after 8 and 2 h, respectively. TGF-beta1 also decreased IP3R mRNA expression by 59% after 1 h. Phosphorylation of the IP3R was also demonstrated as early as 15 min after TGF-beta1 exposure. Back phosphorylation assays of IP3R from TGF-beta1-treated mesangial cells with protein kinase A (PKA), indicated that TGF-beta1-induced phosphorylation of the IP3R occurs at similar sites as for PKA. In vitro kinase assays using the known IP3R peptide substrates for PKA, RPSGRRESLTSFGNP and ARRDSVLAAS, demonstrated that TGF-beta1 induces phosphorylation of both peptides (158 and 123% of control values, respectively). TGF-beta1-induced phosphorylation was prevented by ...Continue Reading

References

Jul 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·T NakagawaK Mikoshiba
Apr 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·S K DanoffC A Ross
Jul 11, 1989·Nucleic Acids Research·T FuruichiK Mikoshiba
Nov 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·S SupattaponeS H Snyder
Apr 1, 1994·Journal of Molecular and Cellular Cardiology·K IizukaA Kitabatake
Jan 1, 1993·Clinical Immunology and Immunopathology·T NakamuraH Koide
Jan 28, 1993·Nature·M J Berridge
Jun 28, 1996·The Journal of Biological Chemistry·R S WymoreK G Chandy
Jan 1, 1996·Cellular Signalling·S K Joseph

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Citations

May 1, 1992·Parasitology Today·P BastienM Pages
Dec 5, 1998·Biochimica Et Biophysica Acta·S B Shears
May 3, 2003·Biochemical and Biophysical Research Communications·Zemfira Z GizatullinaPontus Aspenström
Mar 10, 2000·Cytokine & Growth Factor Reviews·K Sharma, T A McGowan
Sep 21, 2000·Kidney International. Supplement·M SchifferE P Böttinger
Feb 18, 2004·Molecular and Cellular Biology·Lizhi ZhangDiane M Simeone
May 10, 2008·American Journal of Physiology. Gastrointestinal and Liver Physiology·Huibin YangDiane M Simeone
Mar 26, 2010·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·R Scott DuncanPeter Koulen
May 13, 1999·Proceedings of the National Academy of Sciences of the United States of America·A A GenazzaniD Guerini
Jan 31, 2013·The Journal of Biological Chemistry·Huibin YangDiane M Simeone
Jan 27, 2004·The Journal of Biological Chemistry·Jennifer L GoochHanna E Abboud
Apr 15, 2003·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Leo E DeelmanRobert H Henning
May 9, 1998·The Journal of Biological Chemistry·L WangK Sharma
Jun 16, 2001·American Journal of Physiology. Renal Physiology·A N YusufiJ P Grande
Apr 6, 2002·American Journal of Physiology. Renal Physiology·Tracy A McGowanKumar Sharma
Jul 24, 2003·American Journal of Physiology. Renal Physiology·Kumar SharmaGyorgy Hajnoczky
Dec 1, 2005·The Journal of Biological Chemistry·Cunnigaiper D BhanumathySuresh K Joseph
Jun 3, 2000·American Journal of Physiology. Renal Physiology·K SharmaG Hajnóczky
Aug 11, 2018·Frontiers in Physiology·Ali H EidHaissam Abou-Saleh
Feb 11, 2000·Cell Calcium·C W TaylorS A Morris
Dec 26, 2001·Chemical Reviews·J B Shabb
Sep 18, 2021·Journal of Cell Science·Vikas ArigeDavid I Yule

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