PMID: 11912279Mar 26, 2002Paper

Transforming growth factor-beta1 inhibits steroidogenesis in human trophoblast cells

Molecular Human Reproduction
Shuang LuoC Peng

Abstract

Transforming growth factor-beta (TGF-beta) is an important regulator of placental development and function. In this study, we have investigated the effect of TGF-beta1 on steroidogenesis, as well as its sites of action in the steroidogenic pathway by using a choriocarcinoma cell line, JEG-3, and a normal trophoblast cell line (NPC). The effect of TGF-beta1 on progesterone and estradiol production was evaluated in the absence or presence of a membrane-permeable analogue of cholesterol and some intermediate substrates of steroidogenic enzymes. The effect of TGF-beta1 on P450 aromatase (P450arom) mRNA levels was determined by Northern blot analysis. TGF-beta1 significantly decreased progesterone production in both NPC and JEG-3 cells. The inhibitory effect of TGF-beta1 on progesterone production was reversed by addition of 22R-hydroxycholesterol, a membrane-permeable analogue of cholesterol, or pregnenolone. In JEG-3 cells, TGF-beta1 also inhibited estradiol production when androstenedione, but not estrone, was added to the culture. Estradiol production was too low to be detected in NPC cells. Treatment with TGF-beta1 also suppressed aromatase mRNA levels. This study has demonstrated that TGF-beta1 inhibits progesterone and estrad...Continue Reading

Citations

Nov 21, 2009·Human Reproduction Update·K G PringleC T Roberts
Sep 10, 2005·Reproductive Biology and Endocrinology : RB&E·M Cecilia JohnsonM Angélica Boric
Aug 4, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Rebecca L JonesLois A Salamonsen
Aug 4, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Mei-rong ZhaoYan-ling Wang
Jul 19, 2008·Reproduction : the Official Journal of the Society for the Study of Fertility·Xiaofeng ZhengPaul D Carrière
Nov 16, 2004·Molecular and Cellular Endocrinology·M H F Sullivan
Dec 6, 2006·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Raquel CoyaRosa Señarís
Jul 12, 2005·Human Reproduction Update·E DimitriadisL A Salamonsen
Feb 23, 2019·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Nancy Noyola-MartínezDavid Barrera
May 20, 2004·The Journal of Biological Chemistry·Sadia MunirChun Peng
Dec 2, 2010·Systems Biology in Reproductive Medicine·Ioannis MylonasBarbara Schiessl
Dec 22, 2019·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Désirée ForstnerMartin Gauster

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