Transforming growth factor beta1 targets estrogen receptor signaling in bronchial epithelial cells

Respiratory Research
L Cody SmithTara Sabo-Attwood

Abstract

Sex differences in idiopathic pulmonary fibrosis (IPF) suggest a protective role for estrogen (E2); however, mechanistic studies in animal models have produced mixed results. Reports using cell lines have investigated molecular interactions between transforming growth factor beta1 (TGF-β1) and estrogen receptor (ESR) pathways in breast, prostate, and skin cells, but no such interactions have been described in human lung cells. To address this gap in the literature, we investigated a role for E2 in modulating TGF-β1-induced signaling mechanisms and identified novel pathways impacted by estrogen in bronchial epithelial cells. We investigated a role for E2 in modulating TGF-β1-induced epithelial to mesenchymal transition (EMT) in bronchial epithelial cells (BEAS-2Bs) and characterized the effect of TGF-β1 on ESR mRNA and protein expression in BEAS-2Bs. We also quantified mRNA expression of ESRs in lung tissue from individuals with IPF and identified potential downstream targets of E2 signaling in BEAS-2Bs using RNA-Seq and gene set enrichment analysis. E2 negligibly modulated TGF-β1-induced EMT; however, we report the novel observation that TGF-β1 repressed ESR expression, most notably estrogen receptor alpha (ESR1). Results of th...Continue Reading

References

Nov 10, 1994·The New England Journal of Medicine·W A Border, N A Noble
Aug 10, 1999·Experimental Cell Research·M MäkeläV J Uitto
May 4, 2000·The New England Journal of Medicine·G C BlobeH F Lodish
Sep 14, 2001·The Journal of Biological Chemistry·T MatsudaF Saatcioglu
Dec 26, 2001·Nucleic Acids Research·Ron EdgarAlex E Lash
Jun 20, 2002·Endocrinology·Tetsuya YamamotoTadashi Matsuda
Jul 26, 2002·Lung Cancer : Journal of the International Association for the Study of Lung Cancer·Steen MollerupAage Haugen
Jul 26, 2002·Human Molecular Genetics·Christina K HastonElizabeth L Travis
Aug 15, 2002·The Journal of Biological Chemistry·Claudia FolliRodolfo Berni
Dec 4, 2002·Journal of Cellular Biochemistry·Trevor A Petrel, Robert W Brueggemeier
Dec 10, 2003·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·Andrew Leask, David J Abraham
May 20, 2004·American Journal of Physiology. Renal Physiology·Alecia S LovegrovePascale H Lane
May 28, 2005·The American Journal of Pathology·Mehrnaz Gharaee-KermaniSem H Phan
Jun 11, 2005·Respiratory Research·Hidenori KasaiZhi Zhang
Jun 30, 2005·International Journal of Cancer. Journal International Du Cancer·Ji-Ching LaiHuei Lee
Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·Aravind SubramanianJill P Mesirov
Jul 1, 2006·American Journal of Respiratory and Critical Care Medicine·Ganesh RaghuGerry Oster
Aug 22, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Lisa A BeckCristiana Stellato
Aug 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Kevin K KimHarold A Chapman
Jan 2, 2007·The Journal of Steroid Biochemistry and Molecular Biology·Maricarmen D Planas-Silva, Paul K Waltz
May 5, 2007·American Journal of Respiratory and Critical Care Medicine·Amy L OlsonKevin K Brown
Jul 17, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Brigham C Willis, Zea Borok
Feb 16, 2008·American Journal of Respiratory Cell and Molecular Biology·James W VoltzDarryl C Zeldin
Mar 7, 2008·The European Respiratory Journal·M K HanF J Martinez
Feb 20, 2009·The European Respiratory Journal·Z WangM Raghunath
May 6, 2009·Journal of Cellular and Molecular Medicine·Marisa KarowPeter Neth
Jun 26, 2009·American Journal of Physiology. Renal Physiology·Hyunjin NohHi Bahl Lee

❮ Previous
Next ❯

Citations

Sep 6, 2019·Current Allergy and Asthma Reports·Peng Zhang, Joe Zein
Jul 11, 2019·American Journal of Respiratory and Critical Care Medicine·Sharon ElliotMarilyn K Glassberg
Sep 24, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Roberta Sessa StilhanoRodrigo Portes Ureshino
Jan 23, 2020·International Journal of Molecular Sciences·Willy RoqueFreddy Romero
Dec 5, 2018·Experimental Biology and Medicine·Nathalie Fuentes, Patricia Silveyra
Dec 31, 2020·The Laryngoscope·Satomi OzawaShigeru Hirano
May 1, 2021·Brain Sciences·Ana M Hernández-Vega, Ignacio Camacho-Arroyo
Aug 7, 2021·Clinics in Chest Medicine·Theodoros KarampitsakosArgyris Tzouvelekis

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE100574

Methods Mentioned

BETA
RNA-Seq
biopsy
Assay
Protein Assay
ESR
histone acetylation

Software Mentioned

CFX Manager
GraphPad Prism
GraphPad
ENSEMBL
R
Pathway Studio®
Pathway Studio
RSEM
GSEA
Trimmomatic

Related Concepts

Related Feeds

CFTR Mutant Structural Therapy

Over 1700 different mutations in the CFTR genes have been shown to cause cystic fibrosis. Here is the latest research on structural therapy for CFTR mutants.