Transforming growth factor-beta3 promotes mesenchymal cell proliferation and angiogenesis mediated by the enhancement of cyclin D1, Flk-1, and CD31 gene expression during CL/Fr mouse lip fusion

Birth Defects Research. Part A, Clinical and Molecular Teratology
Noriko MuraokaKazuaki Nonaka


Cleft lip with or without cleft palate is the most common congenital anomaly in the craniofacial region. Knowledge of the molecular mechanisms behind normal lip fusion can contribute to better intervention and improved functional clinical outcome. Transforming growth factor-beta3 (TGF-beta3) has been implicated in lip morphogenesis. Therefore, we hypothesized that TGF-beta3 functions during lip fusion through regulation of angiogenesis and mesenchymal cell cycle progression during early developmental stages. To test this hypothesis we used the CL/Fraser mouse model, which has a high incidence of cleft lip. Lips isolated from embryonic day (ED) 11.5 mouse embryos were allowed to develop in serum-free organ cultures in the presence or absence of TGF-beta3. The lips that developed in these cultures fused in 2 days. During normal development, we detected positive immunoreactions for TGF-beta3 at the site of fusion. We also detected mesenchymal cells that were immunopositive for Flk-1 and CD31, which are markers for endothelial cell precursors. Exogenous TGF-beta3 accelerated lip fusion in culture. This enhancement was associated with an increase in the number of capillary blood vessels in the lips cultured in the presence of TGF-be...Continue Reading


Jan 9, 2008·Birth Defects Research. Part A, Clinical and Molecular Teratology·D M Juriloff, Muriel J Harris
Aug 24, 2013·Arteriosclerosis, Thrombosis, and Vascular Biology·Tony E WalshePatricia A D'Amore
Aug 22, 2018·Chinese Medical Journal·Xiao-Yan XuZheng-Wei Yuan
Jan 24, 2007·American Journal of Physiology. Lung Cellular and Molecular Physiology·Donald MassaroLinda Biadasz Clerch
Feb 24, 2019·International Journal of Molecular Sciences·Wansheng JiangJunxing Yang
Sep 12, 2019·Journal of Cellular and Molecular Medicine·Huachen YuQidong Zhang


Apr 1, 1980·Teratology·D M Juriloff, F C Fraser
May 1, 1995·Cell Biology International·D A Cox
Oct 1, 1994·The Journal of Experimental Zoology·M D'AngeloR M Greene
Sep 1, 1994·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·N AminY Takano
Mar 1, 1994·The Cleft Palate-craniofacial Journal : Official Publication of the American Cleft Palate-Craniofacial Association·O G Silva FilhoG Semb
Mar 3, 1999·Proceedings of the National Academy of Sciences of the United States of America·A C SchuhK Choi
Mar 31, 2000·Nature Medicine·P Carmeliet
Jan 6, 2001·Journal of Pediatric Health Care : Official Publication of National Association of Pediatric Nurse Associates & Practitioners·J C Mitchell, R J Wood
Sep 28, 2002·Journal of Dental Research·K KohamaM Ohishi
Mar 26, 2003·Journal of Dental Research·A R VieiraJ C Murray
Apr 18, 2003·Dental Clinics of North America·Deborah A Redford-BadwalJohn D Frassinelli
Jun 25, 2003·Journal of Dental Research·R HosokawaM Ohishi
Aug 19, 2003·Developmental Biology·Tadashi YamamotoCharles F Shuler
Jan 1, 1954·Plastic and Reconstructive Surgery·R B STARK
Oct 3, 2003·Trends in Cardiovascular Medicine·Marie-Jose GoumansGudrun Valdimarsdottir
Dec 9, 2003·Journal of Biochemistry and Molecular Biology·Myung-Hee KimDong-Seok Nahm
Mar 5, 2004·European Journal of Orthodontics·Martyn T Cobourne

Related Concepts

Recombinant Transforming Growth Factor
Transforming Growth Factor beta
BHLHE22 wt Allele
Angiogenic Process
Organ Culture Techniques
Congenital Neurologic Anomalies
Tumor Angiogenesis
Vascular Endothelial Growth Factor Receptor-2
Transforming growth factor beta3

Related Feeds

Birth Defects

Birth defects encompass structural and functional alterations that occur during embryonic or fetal development and are present since birth. The cause may be genetic, environmental or unknown and can result in physical and/or mental impairment. Here is the latest research on birth defects.