Transforming growth factor β mediates communication of co-cultured human nucleus pulposus cells and mesenchymal stem cells

Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society
Tomasz P LehmannPaweł P Jagodziński

Abstract

Intervertebral disc (IVD) consists of surrounding tissue annulus fibrosus and central nucleus pulposus, which are partially degenerative in scoliotic IVDs. Successful regeneration of scoliotic alterations requires cognition of critical paracrine mediators of cell-to-cell contact in the IVD. In this work, we hypothesized that transforming growth factor β (TGF-β) is involved in the intercellular communication of nucleus pulposus cells (NPCs) and mesenchymal stem cells (MSCs). We observed that in cultured NPCs TGF-β1 stimulated COL1A1 expression, encoding collagen I, and in MSCs stimulated COL1A1 and SOX9 expressions. We subsequently co-cultured NPCs and MSCs together using direct and indirect transwell systems. The expression of miR-140 and miR-145 were decreased in co-cultured NPCs. We observed that direct co-culture system stronger than the indirect system decreased expression of three miRNA. The expression of COL1A1, ACAN, encoding aggrecan, and SOX9 genes was increased in MSCs co-cultured with NPCs. Co-cultures were incubated with two inhibitors of TGF-β type I receptor: SB-431542 and SB-525334. In co-cultured NPCs, SB-431542 and SB-525334 annulated downregulation of miR-140 and miR-145. In MSCs these inhibitors diminished st...Continue Reading

References

Mar 17, 2005·The Journal of Pharmacology and Experimental Therapeutics·Eugene T GrygielkoNicholas J Laping
Oct 15, 2005·Stem Cells·Stephen M RichardsonJudith A Hoyland
Aug 30, 2008·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Masayuki UmedaHirokazu Iida
Sep 17, 2008·Spine·Glenn R Buttermann, Brian P Beaubien
Feb 5, 2014·Journal of Tissue Engineering and Regenerative Medicine·Nikolaos K PaschosKyriacos A Athanasiou
May 16, 2014·Journal of the Royal Society, Interface·Lisa GoersKaren M Polizzi
Feb 25, 2015·Nature Reviews. Rheumatology·Daisuke Sakai, Gunnar B J Andersson
Mar 5, 2015·Cell Proliferation·Zheng LiWilliam Ka Kei Wu
May 12, 2015·Matrix Biology : Journal of the International Society for Matrix Biology·Ian B RobertsonDaniel B Rifkin
Jun 3, 2016·Cold Spring Harbor Perspectives in Biology·Ian B Robertson, Daniel B Rifkin
Sep 2, 2016·Stem Cell Research & Therapy·Jeffrey L SpeesCarl A Gregory
Sep 13, 2016·Genes & Diseases·Yi FengJinxi Wang
Apr 25, 2017·Scoliosis and Spinal Disorders·Arnold Yl WongDino Samartzis
May 27, 2017·Journal of Cellular Physiology·Yu ZhangQuanyi Guo
Oct 19, 2017·ASAIO Journal : a Peer-reviewed Journal of the American Society for Artificial Internal Organs·Hossein MahboudiBahram Kazemi

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Citations

Sep 3, 2020·Journal of Orthopaedic Surgery and Research·Shiyu HuTao Lan
Jul 31, 2019·Current Pain and Headache Reports·Ivan UritsVwaire Orhurhu
Mar 28, 2019·Cellular and Molecular Neurobiology·Jiaying YuanXuehong Liu

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