Transforming Properties of Beta-3 Human Papillomavirus E6 and E7 Proteins.

MSphere
Lucia MinoniMassimo Tommasino

Abstract

The beta human papillomaviruses (HPVs) are subdivided into 5 species (beta-1 to beta-5), and they were first identified in the skin. However, the beta-3 species appears to be more highly represented in the mucosal epithelia than in the skin. Functional studies have also highlighted that beta-3 HPV49 shares some functional similarities with mucosal high-risk (HR) HPV16. Here, we describe the characterization of the in vitro transforming properties of the entire beta-3 species, which includes three additional HPV types: HPV75, HPV76, and HPV115. HPV49, HPV75, and HPV76 E6 and E7 (E6/E7), but not HPV115 E6 and E7, efficiently inactivate the p53 and pRb pathways and immortalize or extend the life span of human foreskin keratinocytes (HFKs). As observed for HR HPV16, cell cycle deregulation mediated by beta-3 HPV E6/E7 expression leads to p16INK4a accumulation, whereas no p16INK4a was detected in beta-2 HPV38 E6/E7 HFKs. As shown for HPV49 E6, HPV75 and HPV76 E6s degrade p53 by an E6AP/proteasome-mediated mechanism. Comparative analysis of cellular gene expression patterns of HFKs containing E6 and E7 from HR HPV16, beta-3 HPV types, and beta-2 HPV38 further highlights the functional similarities of HR HPV16 and beta-3 HPV49, HPV75,...Continue Reading

References

Jun 5, 1998·The Journal of Biological Chemistry·J J ChenE J Androphy
Apr 20, 2005·Oncogene·Desssislava K Dimova, Nicholas J Dyson
Jan 7, 2006·EMBO Reports·Rosita AccardiMassimo Tommasino
Aug 19, 2007·The Journal of Infectious Diseases·Ola ForslundUNKNOWN Viraskin Study Group
May 28, 2008·The British Journal of Dermatology·T WaterboerM Pawlita
Dec 10, 2009·British Journal of Cancer·C Ateenyi-AgabaE Weiderpass
Jul 10, 2010·BMJ : British Medical Journal·Margaret R KaragasUNKNOWN New Hampshire Skin Cancer Study Group
Nov 26, 2010·Cancer Research·Jan Nico Bouwes BavinckUNKNOWN EPI-HPV-UV-CA group
Jan 20, 2011·Proceedings of the National Academy of Sciences of the United States of America·Margaret E McLaughlin-DrubinKarl Münger
May 2, 2012·Proceedings of the National Academy of Sciences of the United States of America·Min Jie Alvin TanPeter M Howley
Jun 19, 2012·Cancer Epidemiology, Biomarkers & Prevention : a Publication of the American Association for Cancer Research, Cosponsored by the American Society of Preventive Oncology·Michelle R IannaconeDana E Rollison
Oct 25, 2012·Nucleic Acids Research·Koenraad Van DoorslaerAlison A McBride
Mar 29, 2013·International Journal of Cancer. Journal International Du Cancer·Shohreh F FarzanMargaret R Karagas
Sep 21, 2013·Proceedings of the National Academy of Sciences of the United States of America·Margaret E McLaughlin-DrubinKarl Munger
Oct 19, 2013·International Journal of Cancer. Journal International Du Cancer·Michelle R IannaconeDana E Rollison
Nov 12, 2013·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Christine M Pierce CampbellAnna R Giuliano
Dec 10, 2013·Seminars in Cancer Biology·Massimo Tommasino
Dec 3, 2014·La Presse médicale·Rosita Accardi, Tarik Gheit
Mar 1, 2015·Virology·Peter M Howley, Herbert J Pfister
May 12, 2015·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Montserrat TorresMassimo Tommasino
Jan 21, 2016·Nature·Denise Martinez-ZapienKatia Zanier
May 11, 2016·Virology·Emily Montosa NunesUNKNOWN HIM Study group
May 25, 2016·Cancer Research·Daniele ViarisioMassimo Tommasino
Jul 19, 2016·The Journal of Infection·Maria Gabriella DonàMassimo Giuliani
Sep 9, 2016·Scientific Reports·Koenraad Van Doorslaer, Alison A McBride
Nov 20, 2016·Virus Research·Massimo Tommasino
May 27, 2017·The Journal of Infectious Diseases·Shalaka S HamprasMassimo Tommasino
Jun 12, 2020·International Journal of Cancer. Journal International Du Cancer·Luisa GalatiMassimo Tommasino

❮ Previous
Next ❯

Citations

Nov 10, 2020·Frontiers in Microbiology·Gennaro AltamuraGiuseppe Borzacchiello

❮ Previous
Next ❯

Datasets Mentioned

BETA
GSE100681

Methods Mentioned

BETA
PCR
flow cytometry
pulldown
fluorescence-activated cell sorter
transfection
electrophoresis
in vitro transcription

Software Mentioned

Enrichr
ArrayTools
BRB
Illumina Genome Studio
MIAME

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.