Transgalactosylating β-galactosidase from probiotic Lactobacillus plantarum MCC2156: production and permeabilization for use as whole cell biocatalyst

Journal of Food Science and Technology
Duraiswamy Gobinath, Siddalingaiya Gurudutt Prapulla

Abstract

Key nutritional factors were optimized for the maximum production of transgalactosylating β-galactosidase from Lactobacillus plantarum MCC2156. Galactose, yeast extract, sodium acetate and manganese sulphate were the most important nutrients affecting β-galactosidase production. Maximum β-galactosidase production (3015 miller units) was obtained by culturing L. plantarum in the optimized fermentation medium containing (w/v) galactose (4 %), yeast extract (2 %), sodium acetate (3 %) and manganese sulphate (0.075 %) with an optimum medium pH of 7.0, after 14 h of incubation at 35 °C. Further, permeabilization of L. plantarum cells using various chemical/ solvents for maximum β-galactosidase activity was performed for use as whole cell biocatalyst. Mixture of ethanol: n-butanol was found to effectively permeabilize the cells with maximum β-galactosidase activity under the following optimum conditions; 1: 1 mixture of ethanol (10 %, v/v): n-butanol (30 %, v/v) with a contact time of 10 min at 28 ± 2 °C.

References

Aug 1, 1977·Applied and Environmental Microbiology·M V Rao, S M Dutta
Feb 24, 2001·Folia Microbiologica·J W Kim, S N Rajagopal
Jun 30, 2005·International Journal of Food Microbiology·C A HsuC C Chou
Jul 6, 2006·Journal of Agricultural and Food Chemistry·Barbara SplechtnaDietmar Haltrich
Feb 24, 2007·Journal of Agricultural and Food Chemistry·C A HsuC C Chou
Sep 1, 2010·Comprehensive Reviews in Food Science and Food Safety·Duarte P M TorresLígia R Rodrigues

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Citations

Aug 2, 2018·Journal of Food Science and Technology·Hye Ji JangHyun-Dong Paik

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