Transgene excision from wheat chromosomes by phage phiC31 integrase.

Plant Molecular Biology
Katja KempeMario Gils

Abstract

The Streptomyces phage phiC31 integrase was tested for its ability to excise transgenic DNA from the wheat genome by site-specific recombination. Plants that stably express phiC31 integrase were crossed to plants carrying a target construct bearing the phiC31 recognition sites, attP and attB. In the progeny, phiC31 recombinase mediates recombination between the att sites of the target locus, which results in excision of the intervening DNA. Recombination events could be identified in 34 independent wheat lines by PCR and Southern blot analysis and by sequencing of the excision footprints. Recombinant loci were inherited to the subsequent generation. The results presented here establish the integrase-att system as a tool for catalysing the precise elimination of DNA sequences from wheat chromosomes.

References

Nov 1, 1991·Molecular & General Genetics : MGG·S Maeser, R Kahmann
Apr 1, 1995·The Plant Journal : for Cell and Molecular Biology·H AlbertD W Ow
Dec 1, 1993·Trends in Genetics : TIG·N J KilbyJ A Murray
May 11, 1993·Nucleic Acids Research·W Baubonis, B Sauer
Feb 15, 1997·Nucleic Acids Research·K ArakiK Yamamura
May 20, 1998·Proceedings of the National Academy of Sciences of the United States of America·H M Thorpe, M C Smith
Sep 29, 1999·Proceedings of the National Academy of Sciences of the United States of America·V SrivastavaD W Ow
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·H LuoT K Hodges
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·A LoonstraJ Jonkers
Apr 26, 2002·Molecular Microbiology·Margaret C M Smith, Helena M Thorpe
Jun 4, 2002·Nature Biotechnology·Peter D Hare, Nam-Hai Chua
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·T. T. C. HoaT. K. Hodges
Feb 27, 2003·Plant Molecular Biology·Eric R CoppoolseMark J J van Haaren
Jul 2, 2003·Plant Cell Reports·L A LyznikY Tao
Aug 28, 2003·Journal of Plant Physiology·Holger Puchta
Nov 20, 2003·Trends in Biotechnology·Larry Gilbertson
Mar 5, 2004·The Plant Journal : for Cell and Molecular Biology·Kerry A LutzPal Maliga
May 14, 2004·Nucleic Acids Research·Matthew C A SmithMargaret C M Smith
Aug 13, 2005·Advances in Genetics·Daniel S Ginsburg, Michele P Calos
Sep 15, 2005·Plant Molecular Biology·Eric R CoppoolseMark J J van Haaren
Jun 8, 2006·Annual Review of Biochemistry·Nigel D F GrindleyPhoebe A Rice
Aug 10, 2006·Plant Molecular Biology·Tarinee TungsuchatPal Maliga
Dec 7, 2006·Plant Biotechnology Journal·Vibha SrivastavaAndrea J Wilson
Feb 13, 2007·Plant Molecular Biology·Chokchai KittiwongwattanaPal Maliga
Mar 14, 2007·Current Opinion in Biotechnology·David W Ow
Apr 5, 2007·Nature Protocols·Bryan G Allen, Daniel L Weeks
Oct 2, 2007·Current Opinion in Biotechnology·Dagmar WirthHansjörg Hauser
Dec 19, 2007·Plant Biotechnology Journal·Mario GilsYuri Gleba
Jul 22, 2008·Methods in Molecular Biology·Johannes Bischof, Konrad Basler
Sep 18, 2008·Plant Cell Reports·Myroslava RubtsovaMario Gils
Jul 9, 2009·International Journal of Plant Genomics·Goetz HenselJochen Kumlehn

❮ Previous
Next ❯

Citations

Oct 26, 2010·Plant Cell Reports·Yueju WangJames G Thomson
Oct 2, 2012·PloS One·Eszter KapusiMario Gils
Jun 21, 2011·Yi chuan = Hereditas·Qing-Wen Ma
May 14, 2014·Proceedings of the National Academy of Sciences of the United States of America·Katja KempeMario Gils
May 27, 2014·Journal of Molecular Biology·Paul C M FoggMargaret C M Smith
Mar 11, 2011·Trends in Biotechnology·Charles KwitC Neal Stewart
Aug 27, 2010·Journal of Plant Physiology·Götz HenselJochen Kumlehn
Apr 12, 2013·The Plant Journal : for Cell and Molecular Biology·Annelies De PaepeAnn Depicker
Jun 17, 2011·Journal of Integrative Plant Biology·David W Ow
Jun 5, 2013·Plant Biotechnology Journal·Yi SangC Neal Stewart
Aug 27, 2013·Insect Biochemistry and Molecular Biology·Dingpei LongZhonghuai Xiang
Oct 10, 2013·Nature Reviews. Genetics·Wusheng LiuC Neal Stewart
Apr 20, 2017·Plant Biotechnology Journal·Min ShaoJames G Thomson
May 24, 2020·Communications Biology·Mayna S GomideElibio Rech
Jan 24, 2020·Experimental Cell Research·James A Birchler, Nathan C Swyers

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacteriophage: Phage Therapy

Phage therapy uses bacterial viruses (bacteriophages) to treat bacterial infections and is widely being recognized as an alternative to antibiotics. Here is the latest research.