Transgene expression in mouse airway epithelium by aerosol gene therapy with PEI-DNA complexes

Molecular Therapy : the Journal of the American Society of Gene Therapy
A GautamJ C Waldrep

Abstract

Gene therapy targeted at the respiratory epithelium holds therapeutic potential for diseases such as cystic fibrosis and alpha-1 anti-trypsin deficiency. A variety of approaches such as intranasal or intratracheal instillation and aerosol delivery have been utilized to target genes to the airways. Polyethylenimine (PEI), a linear or branched polycationic polymer, has been used for delivery of genes to various organs. In this study, using fluorescein isothiocyanate (FITC)-labeled branched PEI, we initially examined the localization of PEI in the lungs after aerosol delivery to Balb/C mice. Further, after aerosol delivery of PEI-CAT DNA, in situ immunostaining for chloramphenicol acetyl transferase (CAT) protein was used to localize the transgene expression within the lungs. Immunohistochemistry for CAT, as well as localization of FITC-labeled PEI, revealed that after aerosol delivery, the PEI-DNA complexes deposit and subsequently transfect most of the epithelial cells in the conducting airways (including the peripheral airways). High levels of CAT were detected at 24 h after aerosol exposure and significant CAT expression was detected in the lungs up to 28 days after a single aerosol exposure. The data suggest that aerosol deli...Continue Reading

References

Dec 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·R StriblingR Debs
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·O BoussifJ P Behr
Apr 10, 1996·Human Gene Therapy·L A SchwarzJ C Waldrep
Jul 1, 1997·Human Gene Therapy·A BolettaL Monaco
Nov 5, 1999·Biochimica Et Biophysica Acta·S FerrariM Scarpa
Mar 30, 2000·The Journal of Gene Medicine·N ErnstJ Rosenecker
Jul 19, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·A GautamJ C Waldrep
Aug 10, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·C L DensmoreV Knight
Oct 6, 2000·Molecular Therapy : the Journal of the American Society of Gene Therapy·A GautamJ C Waldrep

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Citations

Jan 15, 2004·American Journal of Respiratory Medicine : Drugs, Devices, and Other Interventions·Ajay GautamCharles L Densmore
Dec 13, 2006·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Petra DamesCarsten Rudolph
Jul 5, 2008·Clinical Reviews in Allergy & Immunology·Christian Mueller, Terence R Flotte
Sep 3, 2002·Advanced Drug Delivery Reviews·Thomas MerdanThomas Kissel
Nov 30, 2002·Advanced Drug Delivery Reviews·Stefano FerrariEric W F W Alton
Jun 12, 2008·The Journal of Biological Chemistry·Hatice Z OzsoyDouglas L Mann
Jul 8, 2005·Human Gene Therapy·Léa DesigauxBruno Pitard
Sep 27, 2007·Journal of Aerosol Medicine : the Official Journal of the International Society for Aerosols in Medicine·J LynchColin Birkinshaw
Oct 2, 2009·Journal of the Royal Society, Interface·Maria Iris HermannsC James Kirkpatrick
Aug 7, 2009·Journal of Biomechanical Engineering·Todd O PangburnEfrosini Kokkoli
Mar 4, 2008·American Journal of Respiratory and Critical Care Medicine·Cheng-Xiong XuMyung-Haing Cho
Mar 17, 2009·American Journal of Respiratory and Critical Care Medicine·Soon-Kyung HwangMyung-Haing Cho
Nov 19, 2009·Drug Design, Development and Therapy·Sante Di Gioia, Massimo Conese
Oct 31, 2012·International Journal of Molecular Sciences·Paul ZarogouldisKonstantinos Zarogoulidis
Jul 1, 2010·Therapeutic Delivery·Corinna PfeiferCarsten Rudolph
Mar 20, 2003·Proceedings of the National Academy of Sciences of the United States of America·Junghae SuhJustin Hanes
Jul 2, 2005·Expert Opinion on Emerging Drugs·D C Górecki
Sep 29, 2009·Expert Opinion on Drug Delivery·Andrew J DittoYang H Yun
May 20, 2009·Expert Opinion on Drug Delivery·Manish K AnejaCarsten Rudolph
Oct 2, 2003·Expert Opinion on Biological Therapy·Charles L Densmore
Jun 5, 2003·Expert Opinion on Biological Therapy·Assem G ZiadyMichael W Konstan
Dec 8, 2009·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Eugenia LesinaCarsten Rudolph
Sep 20, 2008·Journal of Controlled Release : Official Journal of the Controlled Release Society·Stéphanie GrosseIsabelle Fajac
Jan 19, 2008·Peptides·Guillaume MorissetteFrançois Marceau
Mar 17, 2010·Microscopy Research and Technique·Yuguo LeiTatiana Segura
Nov 30, 2004·Journal of Controlled Release : Official Journal of the Controlled Release Society·L A DaileyT Kissel
Jun 23, 2004·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Maytal Bivas-BenitaGerrit Borchard
Nov 22, 2005·Journal of Controlled Release : Official Journal of the Controlled Release Society·E KleemannT Kissel
Jun 9, 2005·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·U LungwitzA Göpferich
Nov 12, 2013·Biomaterials·Talar TokatlianTatiana Segura
Oct 8, 2011·International Journal of Pharmaceutics·Z MohammadiM Rafiee Tehrani
Nov 26, 2010·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Melanie GüntherAchim Aigner

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