Transgene expression in strawberries driven by a heterologous phloem-specific promoter

Plant Cell Reports
Yan ZhaoRobert E Davis

Abstract

Strawberry is susceptible to diseases caused by phytoplasmas, mycoplasma-like prokaryotes restricted to sieve elements in the phloem tissue of infected plants. One strategy to improve strawberry resistance to phytoplasmas involves transgenic expression of anti-microbial peptide genes in phloem. For targeted phloem-specific expression, we constructed a binary vector with an expression cassette bearing the beta-glucuronidase (GUS) reporter gene (uidA) under control of the Arabidopsis sucrose-H+ symporter gene (AtSUC2) promoter. Transgenic strawberry lines were generated with high efficiencies by a modified transformation protocol, which combines the adoption of a 3-day pre-selection period following transformation, and the addition of 10-microM thidiazuron to the regeneration medium. Histological GUS activity indicated that the reporter gene was expressed specifically in phloem of leaves, petioles, and roots of transgenic plants. The results suggest that the transformation protocol and the AtSUC2 promoter may be useful for engineering phytoplasma-resistant transgenic strawberries.

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Citations

Feb 21, 2007·Plant Cell Reports·Andrew E NewhouseWilliam A Powell
Jul 18, 2012·Plant Cell Reports·Luzia Yuriko MiyataFrancisco de Assis Alves Mourão Filho
Mar 3, 2012·Transgenic Research·Giorgio Gambino, Ivana Gribaudo
Jan 5, 2012·Critical Reviews in Food Science and Nutrition·Andrés Cruz-Hernández, Octavio Paredes-López
Feb 19, 2008·Biotechnology Advances·Yonghua QinShanglong Zhang
Aug 30, 2011·Biotechnology Advances·Mohammad Reza SafarnejadStefan Schillberg
Dec 11, 2013·GM Crops & Food·Hossam ZakariaNaglaa A Abdallah
Dec 20, 2011·GM Crops·Amjad Masood HusainiBushra Aquil
Dec 3, 2014·Plant Science : an International Journal of Experimental Plant Biology·Dhirendra Kumar
Jan 1, 2014·Horticulture Research·Manjul DuttJude W Grosser

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