Apr 15, 2015

Transgene expression in the Nop-tTA driver line is not inherently restricted to the entorhinal cortex

Brain Structure & Function
Michael J YetmanJoanna L Jankowsky

Abstract

The entorhinal cortex (EC) plays a central role in episodic memory and is among the earliest sites of neurodegeneration and neurofibrillary tangle formation in Alzheimer's disease. Given its importance in memory and dementia, the ability to selectively modulate gene expression or neuronal function in the EC is of widespread interest. To this end, several recent studies have taken advantage of a transgenic line in which the tetracycline transactivator (tTA) was placed under control of the neuropsin (Nop) promoter to limit transgene expression within the medial EC and pre-/parasubiculum. Although the utility of this driver is contingent on its spatial specificity, no detailed neuroanatomical analysis of its expression has yet been conducted. We therefore undertook a systematic analysis of Nop-tTA expression using a lacZ reporter and have made the complete set of histological sections available through the Rodent Brain Workbench tTA atlas, www.rbwb.org . Our findings confirm that the highest density of tTA expression is found in the EC and pre-/parasubiculum, but also reveal considerable expression in several other cortical areas. Promiscuous transgene expression may account for the appearance of pathological protein aggregates ou...Continue Reading

Mentioned in this Paper

Tetracycline Antibiotics
Establishment and Maintenance of Localization
Body Regions
Study
Tetracyclines
Genes
Neurofibrillary Degeneration (Morphologic Abnormality)
5-bromo-4-chloro-3-indolyl beta-galactoside
Spatial Distribution
Brain

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