PMID: 9191082Jun 1, 1997Paper

Transgene expression of plasmid DNAs directed by viral or neural promoters in the rat brain

Brain Research. Molecular Brain Research
Z Hannas-DjebbaraB Jacquemont

Abstract

The use of circular plasmid DNA may be an alternative method for the transfer of genes into the brain and is presumably easier to use than other vectors, such as viruses or genetically engineered cells. The effectiveness and time course of the expression of a reporter gene (LacZ), directed by appropriate promoters, was studied after stereotaxic injection of naked plasmid DNAs into the rat thalamus, cortex or cerebellum. The efficiencies of three different promoters, the human cytomegalovirus (HCMV) promoter and the glial fibrillary acidic protein (GFAP) and neuron-specific enolase (NSE) promoters (specific for astrocytes and neurons, respectively) to drive reporter gene expression were compared. Efficient expression of beta-gal, detected by X-gal histochemistry or immunochemistry, required the use of 50 microg of DNA and was detectable as early as 48 h after injection. Expression increased until day 8, remained stable until day 15, then decreased over 2 months, probably as a result of non-specific degradation of the plasmids within the transfected cells rather than from specific down-regulation of promoters, as the same time course was seen with all three promoters tested. Depending on the promoter used (GFAP or NSE), LacZ was ...Continue Reading

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Citations

Sep 22, 2007·European Journal of Nuclear Medicine and Molecular Imaging·Do Won HwangDong Soo Lee
Feb 8, 2003·Neuroscience Research·Keishi SekiguchiRyuichi Morishita
Apr 14, 2000·Brain Research. Molecular Brain Research·D FrechillaJ Del Río
Oct 22, 2003·Neurosurgery Clinics of North America·Mary E Garrity-MosesNicholas M Boulis
Mar 16, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·P M Schulte
Aug 12, 2010·The Cerebellum·Jean-Pierre LouboutinDavid S Strayer
Apr 20, 2004·Biochemical and Biophysical Research Communications·Abesh Kumar BhattacharjeeEiji Kohmura
Apr 23, 2008·Current Protocols in Neuroscience·R L Neve, F Lim
Oct 25, 2012·Current Protocols in Neuroscience·Rachael L Neve

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