Transgene-mediated cosuppression and RNA interference enhance germ-line apoptosis in Caenorhabditis elegans.

Proceedings of the National Academy of Sciences of the United States of America
Adele AdamoAdriana La Volpe

Abstract

Introduction of multiple copies of a germ-line-expressed gene elicits silencing of the corresponding endogenous gene during Caenorhabditis elegans oogenesis; this process is referred to as germ-line cosuppression. Transformed plasmids assemble into extrachromosomal arrays resembling extra minichromosomes with repetitive structures. Loss of the transgene extrachromosomal array leads to reversion of the silencing phenomenon. Cosuppression and RNAi depend upon some of the same genes. In the C. elegans germ line, about half the cells undergo a physiological programmed cell death that shares most genetic requirements with somatic apoptosis. In addition, apoptosis is stimulated by DNA damage and synaptic failure mediated through different apoptotic checkpoints. We found that both germ-line cosuppression and RNAi of germ-line-expressed genes enhance apoptosis during C. elegans oogenesis. In contrast, apoptosis is not enhanced by extrachromosomal arrays carrying genes not driven by germ-line-specific promoters that thus do not elicit transgene-mediated cosuppression/silencing. Similarly, introduction of doubled-stranded RNA that shares no homology with endogenous genes has no effect on apoptosis. "Silencing-induced apoptosis" is depend...Continue Reading

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Citations

Aug 13, 2014·Proceedings of the National Academy of Sciences of the United States of America·Cheng-Gang ZouKe-Qin Zhang
May 6, 2015·Proceedings of the National Academy of Sciences of the United States of America·Luciana E LeopoldShawn Ahmed
Sep 24, 2013·Worm·Adele Adamo, Adriana La Volpe
Jun 10, 2018·Genetics·Marcello Germoglio, Adele Adamo
Aug 7, 2020·Essays in Biochemistry·Zhongyang Lin, Karen Wing Yee Yuen
Dec 3, 2020·Scientific Reports·Pamela SantonicolaAdele Adamo

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Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis