Transgenerational DNA Methylation Changes in Daphnia magna Exposed to Chronic γ Irradiation

Environmental Science & Technology
Marie TrijauFrédéric Alonzo

Abstract

Our aim was to investigate epigenetic changes in Daphnia magna after a 25-day chronic external γ irradiation (generation F0 exposed to 6.5 μGy·h-1 or 41.3 mGy·h-1) and their potential inheritance by subsequent recovering generations, namely, F2 (exposed as germline cells in F1 embryos) and F3 (the first truly unexposed generation). Effects on survival, growth, and reproduction were observed and DNA was extracted for whole-genome bisulfite sequencing in all generations. Results showed effects on reproduction in F0 but no effect in the subsequent generations F1, F2, and F3. In contrast, we observed significant methylation changes at specific CpG positions in every generation independent of dose rate, with a majority of hypomethylation. Some of these changes were shared between dose rates and between generations. Associated gene functions included gene families and genes that were previously shown to play roles during exposure to ionizing radiation. Common methylation changes detected between generations F2 and F3 clearly showed that epigenetic modifications can be transmitted to unexposed generations, most likely through the germline, with potential implications for environmental risk.

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Citations

Feb 12, 2020·Biological Reviews of the Cambridge Philosophical Society·Guilherme JeremiasJana Asselman
Mar 10, 2019·BMC Genomics·Jack HearnTom J Little
Oct 6, 2018·Environmental Health and Toxicology·Nivedita ChatterjeeJinhee Choi
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Oct 22, 2019·Current Opinion in Neurobiology·Noa LibermanEric Lieberman Greer
Oct 16, 2020·Journal of Environmental Radioactivity·Kewin GombeauChristelle Adam-Guillermin
Nov 19, 2020·Genesis : the Journal of Genetics and Development·Nhan Duc NguyenHajime Watanabe
May 29, 2021·Aquatic Toxicology·Rikke PoulsenNina Cedergreen
Aug 17, 2018·Environmental Science & Technology·Guilherme JeremiasJana Asselman

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