Transgenerational effects of prenatal restricted diet on gene expression and histone modifications in the rat

PloS One
Joanna Nowacka-WoszukAgata Chmurzynska

Abstract

Dietary triggers acting on a developing fetus can affect the functioning of the body in later life; this can be observed on various levels, including epigenetic modifications and gene expression. Early-life programmed changes may be transmitted to successive generations. In this study, the impact of prenatal restricted diet was studied in four generations of rats. We hypothesized that this diet can induce changes in the expression of major genes involved in two epigenetic mechanisms: DNA methylation and histone modifications. The transcript level of six genes involved in these processes (Dnmt1, Dnmt3a, Dnmt3b, Mecp2, Hdac1, and Sin3a) was therefore determined in three tissues (liver, adipose, and muscle). This diet was found to have no effect on the F0 pregnant females. In the F1 progeny (fetuses at day 19 of pregnancy and 4-week-old rats) significant differences in the expression of the genes were observed mostly in the liver; in subsequent generations, we therefore studied only this tissue. Among the genes encoding DNA methyltransferases, significant changes were observed for Dnmt1 in the F1 animals from the restricted group, but these were no longer evident in F2 and F3. The Dnmt3a and Dnmt3b genes showed no differences in m...Continue Reading

Citations

Mar 26, 2019·The Journal of Endocrinology·Folami Y IderaabdullahCatherine Ann Peterson
Nov 2, 2019·Journal of Applied Genetics·Joanna Nowacka-Woszuk
Mar 28, 2019·Frontiers in Genetics·Diego Molina-SerranoAntonis Kirmizis
May 1, 2020·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Daniela KasparJohannes Beckers

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Methods Mentioned

BETA
acetylation
PCR
electrophoresis
Protein Assay
between
histone acetylation

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