Transgenerational lipid-reducing activity of benzylisoquinoline alkaloids in Caenorhabditis elegans

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
Yit-Lai Chow, Fumihiko Sato

Abstract

Epigenetic mechanisms allow for transgenerational memory of an ancestor's environment and can affect the gene expression, physiology and phenotype of that ancestor's descendants, independent of DNA sequence alteration. Among many model organisms, Caenorhabditis elegans has been instrumental in studies of transgenerational inheritance, most of which have focused on the effects of external stressors of the parent worm on the life span and stress resistance of future generations. In this work, we used Nile red staining of accumulated lipids in C. elegans to investigate the transgenerational effect of two benzylisoquinoline alkaloids, namely, berberine and sanguinarine. Our results showed that a reduction in Nile red fluorescence can be propagated to subsequent worm generations. Using mutant worms, we found that the transgenerational effect requires the ASH-2 component of the histone H3K4me3 complex and the HRDE-1 worm Argonaute protein. Ash-2 is also required for transgenerational inheritance of the xenobiotic response in the worm. Our study offers new insights into transmissible drug effects across multiple generations and suggests the importance of such analyses in the drug development process.

References

Oct 9, 1987·Science·R Holliday
Aug 1, 1995·Proceedings of the National Academy of Sciences of the United States of America·Gordon J LithgowT E Johnson
Nov 1, 1994·Journal of Gerontology·Gordon J LithgowT E Johnson
May 28, 2004·Nature·Gerda EggerPeter A Jones
May 25, 2005·Archives of Biochemistry and Biophysics·Ralph MenzelChristian E W Steinberg
Aug 12, 2006·Journal of Experimental Zoology. Part A, Comparative Experimental Biology·Tim H Lindblom, Allyn K Dodd
Apr 26, 2007·European Journal of Human Genetics : EJHG·Gunnar KaatiMichael Sjöström
Dec 1, 2007·WormBook : the Online Review of C. Elegans Biology·Theresa Stiernagle
Jun 5, 2008·Cell Metabolism·Supriya SrinivasanKaveh Ashrafi
Jan 24, 2009·Proceedings of the National Academy of Sciences of the United States of America·Shuzhen ChenYang Shi
Oct 22, 2009·PloS One·Kyleann K BrooksJennifer L Watts
Nov 4, 2009·Cell Metabolism·Eyleen J O'RourkeGary Ruvkun
Oct 26, 2010·Current Biology : CB·Jean-Jacques Remy
Dec 15, 2010·BMC Cell Biology·Shaobing O ZhangHo Yi Mak
Mar 11, 2011·Nature Chemical Biology·George A LemieuxZena Werb
May 31, 2011·Free Radical Biology & Medicine·Michael Ristow, Sebastian Schmeisser
Apr 19, 2012·PLoS Genetics·Pranali P PathareMarc R Van Gilst
Aug 2, 2012·The EMBO Journal·Maartje J LuteijnRené F Ketting
Nov 23, 2012·G3 : Genes - Genomes - Genetics·C. elegans Deletion Mutant Consortium
Feb 16, 2013·Trends in Genetics : TIG·Jana P Lim, Anne Brunet
Apr 11, 2013·Ageing Research Reviews·Malgorzata LagiszShinichi Nakagawa
Dec 10, 2013·Bioscience, Biotechnology, and Biochemistry·Yit-Lai Chow, Fumihiko Sato
Apr 1, 2014·Cell·Edith Heard, Robert A Martienssen
Jan 2, 2015·Scientific Reports·Daniel SchottCraig P Hunter
Dec 5, 2017·ACS Omega·Yit-Lai ChowFumihiko Sato

Citations

Feb 24, 2019·Environmental Science and Pollution Research International·Shuhao DaiLihong Yin
Sep 13, 2020·Hormones and Behavior·Krassimira A GarbettJ David Sweatt

Related Concepts

Sanguinarine sulfate (1: 1)
Nile red
Plant alkaloid
Metazoa
Antilipemic agent
Berberine
Subfecundity
Fluorescence
Isoquinolines
Oxazines

Related Feeds

Anthelmintics (ASM)

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.

Anthelmintics

Anthelmintics or antihelminthics are a group of antiparasitic drugs that expel parasitic worms (helminths) and other internal parasites from the body by either stunning or killing them and without causing significant damage to the host. Discover the latest research on anthelmintics here.