Transgenesis via permanent integration of genes in repopulating spermatogonial cells in vivo

Nature Methods
Suveera Dhup, Subeer S Majumdar


Current techniques for making transgenic mice are cumbersome, requiring trained personnel, costly infrastructure and collection of many zygotes from mice that are then killed. We developed a reproducible nonterminal technique for transfecting genes in undifferentiated spermatogonia through in vivo electroporation of the testis; about 94% of male mice electroporated with different transgenes successfully sired transgenic pups. Such electroporated males provide a valuable resource for continuous production of transgenic founders for more than a year.


Jun 1, 1997·Molecular Biotechnology·E R Cameron
Jan 5, 2000·The Journal of Experimental Zoology·Y YamazakiK Imoto
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M NaganoR L Brinster
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mito Kanatsu-ShinoharaTakashi Shinohara
Jun 22, 2002·Science·R L Brinster
Jun 11, 2004·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Oct 1, 2004·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Feb 17, 2005·Journal of Andrology·Yukihiro UmemotoKenjiro Kohri
Aug 10, 2007·Biology of Reproduction·Jonathan R YehM Nagano


Dec 2, 2009·Cell Biology and Toxicology·Dominic J Wells
Feb 5, 2013·Progress in Biophysics and Molecular Biology·Subeer S Majumdar, Indrashis Bhattacharya
Aug 8, 2012·The Journal of Experimental Medicine·Hridesh BanerjeeSatyajit Rath
Oct 18, 2011·Asian Journal of Andrology·Zhen ZhangFei Zhou
Jul 27, 2012·Cellular and Molecular Life Sciences : CMLS·Xiangyang Miao
Oct 27, 2015·The Journal of Reproduction and Development·Pedro MoreiraAlfonso Gutiérrez-Adán
Jul 4, 2012·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Xin HeWei Li
Dec 26, 2015·Journal of Animal Science and Biotechnology·Jinzhou QinWenxian Zeng
Nov 3, 2009·Journal of Reproductive Immunology·Subeer S MajumdarSuveera Dhup
Apr 26, 2016·Molecular Therapy. Nucleic Acids·Bhola Shankar Pradhan, Subeer S Majumdar
Dec 7, 2013·Scientific Reports·Abul UsmaniSubeer S Majumdar
Oct 9, 2009·Biology of Reproduction·Chong LiTeruhiko Wakayama
Dec 4, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anil ChandrashekranCarol Readhead
Jun 21, 2013·Alternatives to Laboratory Animals : ATLA·Elisabeth H OrmandyGilly Griffin

Related Concepts

Enhanced green fluorescent protein
Cell Differentiation Process
Founder Mice, Transgenic
Proteins, Recombinant DNA
Poly(A) Tail
Gene Expression
Nested Polymerase Chain Reaction

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.