Transgenesis via permanent integration of genes in repopulating spermatogonial cells in vivo

Nature Methods
Suveera Dhup, Subeer S Majumdar

Abstract

Current techniques for making transgenic mice are cumbersome, requiring trained personnel, costly infrastructure and collection of many zygotes from mice that are then killed. We developed a reproducible nonterminal technique for transfecting genes in undifferentiated spermatogonia through in vivo electroporation of the testis; about 94% of male mice electroporated with different transgenes successfully sired transgenic pups. Such electroporated males provide a valuable resource for continuous production of transgenic founders for more than a year.

References

Jun 1, 1997·Molecular Biotechnology·E R Cameron
Jan 5, 2000·The Journal of Experimental Zoology·Y YamazakiK Imoto
Oct 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·M NaganoR L Brinster
Jan 31, 2002·Proceedings of the National Academy of Sciences of the United States of America·Mito Kanatsu-ShinoharaTakashi Shinohara
Jun 22, 2002·Science·R L Brinster
Jun 11, 2004·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Oct 1, 2004·Biology of Reproduction·Mito Kanatsu-ShinoharaTakashi Shinohara
Feb 17, 2005·Journal of Andrology·Yukihiro UmemotoKenjiro Kohri
Aug 10, 2007·Biology of Reproduction·Jonathan R YehM Nagano

Citations

Dec 2, 2009·Cell Biology and Toxicology·Dominic J Wells
Feb 5, 2013·Progress in Biophysics and Molecular Biology·Subeer S Majumdar, Indrashis Bhattacharya
Aug 8, 2012·The Journal of Experimental Medicine·Hridesh BanerjeeSatyajit Rath
Oct 18, 2011·Asian Journal of Andrology·Zhen ZhangFei Zhou
Jul 27, 2012·Cellular and Molecular Life Sciences : CMLS·Xiangyang Miao
Oct 27, 2015·The Journal of Reproduction and Development·Pedro MoreiraAlfonso Gutiérrez-Adán
Jul 4, 2012·Journal of Genetics and Genomics = Yi Chuan Xue Bao·Xin HeWei Li
Dec 26, 2015·Journal of Animal Science and Biotechnology·Jinzhou QinWenxian Zeng
Nov 3, 2009·Journal of Reproductive Immunology·Subeer S MajumdarSuveera Dhup
Apr 26, 2016·Molecular Therapy. Nucleic Acids·Bhola Shankar Pradhan, Subeer S Majumdar
Dec 7, 2013·Scientific Reports·Abul UsmaniSubeer S Majumdar
Oct 9, 2009·Biology of Reproduction·Chong LiTeruhiko Wakayama
Dec 4, 2013·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Anil ChandrashekranCarol Readhead
Jun 21, 2013·Alternatives to Laboratory Animals : ATLA·Elisabeth H OrmandyGilly Griffin

Related Concepts

Enhanced green fluorescent protein
Cell Differentiation Process
Founder Mice, Transgenic
Proteins, Recombinant DNA
Poly(A) Tail
Spermatogonia
Testis
Transfection
Gene Expression
Nested Polymerase Chain Reaction

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