Transgenic Arabidopsis Plants Expressing Tomato Glutathione S-Transferase Showed Enhanced Resistance to Salt and Drought Stress

PloS One
Jing XuQuan-Hong Yao

Abstract

Although glutathione S-transferases (GST, EC 2.5.1.18) are involved in response to abiotic stress, limited information is available regarding gene function in tomato. In this study, a GST gene from tomato, designated LeGSTU2, was cloned and functionally characterized. Expression profile analysis results showed that it was expressed in roots and flowers, and the transcription was induced by salt, osmotic, and heat stress. The gene was then introduced to Arabidopsis by Agrobacterium tumefaciens-mediated transformation. Transgenic Arabidopsis plants were normal in terms of growth and maturity compared with wild-type plants. Transgenic plants also showed an enhanced resistance to salt and osmotic stress induced by NaCl and mannitol. The increased tolerance of transgenic plants was correlated with the changes in proline, malondialdehyde and antioxidative emzymes activities. Our results indicated that the gene from tomato plays a positive role in improving tolerance to salinity and drought stresses in Arabidopsis.

References

Jun 23, 1999·The Plant Journal : for Cell and Molecular Biology·I CumminsR Edwards
Aug 6, 2000·The Plant Journal : for Cell and Molecular Biology·Y SaijoK Izui
Feb 15, 2001·Trends in Plant Science·J K Zhu
Sep 12, 2002·Annual Review of Plant Biology·Jian-Kang Zhu
Sep 18, 2002·Trends in Plant Science·Ron Mittler
Feb 13, 2003·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·H. KanzakiI. Nakamura
Jun 1, 1996·Annual Review of Plant Physiology and Plant Molecular Biology·Kathleen A. Marrs
Oct 6, 2007·Journal of Experimental Botany·Yuanqing JiangMichael K Deyholos
Mar 13, 2009·Plant Physiology·David A DaltonChristopher G Taylor
Oct 15, 2010·Bioscience, Biotechnology, and Biochemistry·Md Anamul HoqueYoshiyuki Murata
Dec 21, 2010·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Yunhua ChiShu-Ye Jiang
Mar 24, 2011·Drug Metabolism Reviews·Ian CumminsRobert Edwards
Jan 28, 2012·Journal of Experimental Botany·Saijaliisa KangasjärviGraham Noctor
Jan 1, 2010·The Arabidopsis Book·David P Dixon, Robert Edwards

❮ Previous
Next ❯

Citations

Feb 11, 2016·Biochimica Et Biophysica Acta·Patricie JohnováBřetislav Brzobohatý
Oct 21, 2016·International Journal of Molecular Sciences·Xiaoli WangShaojun Dai
Aug 10, 2019·G3 : Genes - Genomes - Genetics·Silvia MadritschEva M Sehr
Feb 12, 2020·International Journal of Molecular Sciences·Bhakti PrinsiLuca Espen
Oct 6, 2019·Scientific Reports·Daniela PontiggiaBenedetta Mattei
Aug 23, 2017·Molecular Biotechnology·Praveen GuleriaShiwani Guleria
Aug 29, 2019·Frontiers in Microbiology·Raffaella BalestriniIsabella Pentimone
Jun 23, 2018·Frontiers in Plant Science·Smita Kumar, Prabodh K Trivedi
Nov 30, 2019·International Journal of Molecular Sciences·Zhilei JiangYidan Li
Nov 8, 2018·International Journal of Molecular Sciences·Mei ZhangShu-Guang Jian
Aug 21, 2019·Genetics and Molecular Biology·Wendell Jacinto PereiraRosana Pereira Vianello
Jul 14, 2020·3 Biotech·Swati VaishMahesh Kumar Basantani
Apr 11, 2018·Scientific Reports·Anna MichalettiSara Rinalducci
Jun 2, 2017·Frontiers in Plant Science·Yiwei JiangDavid F Garvin
Feb 26, 2019·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Zhe WangHeping Cao
Dec 31, 2020·International Journal of Molecular Sciences·Lisa DavidSixue Chen
Mar 4, 2021·International Journal of Molecular Sciences·Evangelia StavridouIrini Nianiou-Obeidat
May 1, 2021·Journal of Fungi·Murugesan ChandrasekaranParamasivan Manivannan
Sep 6, 2017·Journal of Agricultural and Food Chemistry·Liu WangLin Shen
Oct 12, 2021·Frontiers in Plant Science·Pedro Augusto Medeiros BarbosaTerence Luke Molnar

❮ Previous
Next ❯

Datasets Mentioned

BETA
AY082341
BT012695
U41998

Methods Mentioned

BETA
transgenic
PCR
MDA

Software Mentioned

Compute pI / Mw
Mega
Clustal W2

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.