Transgenic banana plants expressing small interfering RNAs targeted against viral replication initiation gene display high-level resistance to banana bunchy top virus infection

The Journal of General Virology
Upendra K S ShekhawatAshok B Hadapad

Abstract

The banana aphid-transmitted Banana bunchy top virus (BBTV) is the most destructive viral pathogen of bananas and plantains worldwide. Lack of natural sources of resistance to BBTV has necessitated the exploitation of proven transgenic technologies for obtaining BBTV-resistant banana cultivars. In this study, we have explored the concept of using intron-hairpin-RNA (ihpRNA) transcripts corresponding to viral master replication initiation protein (Rep) to generate BBTV-resistant transgenic banana plants. Two ihpRNA constructs namely ihpRNA-Rep and ihpRNA-ProRep generated using Rep full coding sequence or Rep partial coding sequence together with its 5' upstream regulatory region, respectively, and castor bean catalase intron were successfully transformed into banana embryogenic cells. ihpRNA-Rep- and ihpRNA-ProRep-derived transgenic banana plants, selected based on preliminary screening for efficient reporter gene expression, were completely resistant to BBTV infection as indicated by the absence of disease symptoms after 6 months of viruliferous aphid inoculation. The resistance to BBTV infection was also evident by the inability to detect cDNAs coding for viral coat protein, movement protein and Rep protein by RT-PCR from inoc...Continue Reading

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Citations

Jul 16, 2014·Plant Molecular Biology·Manish TiwariPrabodh Kumar Trivedi
Sep 19, 2014·BMC Research Notes·Tuong Vi T DangSerge Remy
Jan 29, 2016·Trends in Plant Science·Yves DessauxDenis Faure
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Jul 5, 2017·Molecular Biotechnology·Poonam BhartiSudesh Kumar Yadav
Mar 14, 2019·3 Biotech·Siddhesh B Ghag, Thumballi R Ganapathi
Jul 26, 2019·Viruses·Fatima Yousif Gaffar, Aline Koch
Oct 17, 2019·Plant Biotechnology Journal·Yaling ZhaoTong Zhang
Sep 25, 2019·Plants·Khushwant SinghJiban Kumar Kundu
Jul 11, 2018·Transgenic Research·Fatima NaimJames Dale
Nov 10, 2017·Emerging Topics in Life Sciences·Jorge Martínez-FortúnHuw D Jones
Jan 1, 2021·Food and Energy Security·Temitope JekayinoluwaJames Dale
Feb 16, 2021·Frontiers in Microbiology·Leena TripathiP Lava Kumar
Jul 19, 2021·Horticulture Research·Maria Lobato-GómezPatricia Sarai Girón-Calva

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