Transgenic biofortification of the starchy staple cassava (Manihot esculenta) generates a novel sink for protein

PloS One
Mohammad AbharyClaude M Fauquet

Abstract

Although calorie dense, the starchy, tuberous roots of cassava provide the lowest sources of dietary protein within the major staple food crops (Manihot esculenta Crantz). (Montagnac JA, Davis CR, Tanumihardjo SA. (2009) Compr Rev Food Sci Food Saf 8:181-194). Cassava was genetically modified to express zeolin, a nutritionally balanced storage protein under control of the patatin promoter. Transgenic plants accumulated zeolin within de novo protein bodies localized within the root storage tissues, resulting in total protein levels of 12.5% dry weight within this tissue, a fourfold increase compared to non-transgenic controls. No significant differences were seen for morphological or agronomic characteristics of transgenic and wild type plants in the greenhouse and field trials, but relative to controls, levels of cyanogenic compounds were reduced by up to 55% in both leaf and root tissues of transgenic plants. Data described here represent a proof of concept towards the potential transformation of cassava from a starchy staple, devoid of storage protein, to one capable of supplying inexpensive, plant-based proteins for food, feed and industrial applications.

References

Nov 1, 1989·Molecular & General Genetics : MGG·M Köster-TöpferL Willmitzer
Jul 1, 1988·Annals of Clinical Biochemistry·M FalkenssonB Sörbo
May 1, 1994·The American Journal of Clinical Nutrition·V R Young, P L Pellett
Aug 31, 1999·The Proceedings of the Nutrition Society·D J Millward
Feb 11, 2000·Toxicon : Official Journal of the International Society on Toxinology·J Vetter
Mar 15, 2000·Proceedings of the National Academy of Sciences of the United States of America·S ChakrabortyA Datta
Jun 7, 2002·Plant Foods for Human Nutrition·V G Sreeja, S Leelamma
Oct 27, 2004·Plant Physiology·Davide MainieriAlessandro Vitale
Jan 5, 2005·Plant Molecular Biology·Dimuth Siritunga, Richard Sayre
Jan 27, 2005·Plant Molecular Biology·Mabrouk A El-Sharkawy
Nov 7, 2007·Genetics and Molecular Research : GMR·D C S Sena, W F Molina
Jul 1, 2009·Comprehensive Reviews in Food Science and Food Safety·Julie A MontagnacSherry A Tanumihardjo

❮ Previous
Next ❯

Citations

Apr 30, 2011·Annual Review of Plant Biology·Richard SayrePeng Zhang
May 27, 2011·Protoplasma·Verena Ibl, Eva Stoger
Jan 10, 2012·Current Opinion in Biotechnology·Elisa Leyva-GuerreroRichard T Sayre
May 14, 2011·Journal of Integrative Plant Biology·Jia LiuPeng Zhang
Apr 21, 2012·Journal of Agricultural and Food Chemistry·Anna Elizabeth BurnsTimothy Richard Cavagnaro

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
genetic modification
PCR
X-ray
scanning

Software Mentioned

ImageQuant
ImagQuant
Analyst QS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Current Opinion in Biotechnology
Elisa Leyva-GuerreroRichard T Sayre
Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology
J M LeggettI P King
© 2021 Meta ULC. All rights reserved