Transgenic Bt rice lines producing Cry1Ac, Cry2Aa or Cry1Ca have no detrimental effects on Brown Planthopper and Pond Wolf Spider

Scientific Reports
Lin NiuWeihua Ma

Abstract

Transgenic rice expressing cry genes from the bacterium Bacillus thuringiensis (Bt rice) is highly resistant to lepidopteran pests. The brown planthopper (BPH, Nilaparvata lugens) is the main non-target sap-sucking insect pest of Bt transgenic rice. The pond wolf spider (PWS, Pardosa pseudoannulata) is one of the most dominant predators of BPH in rice fields. Consequently, the safety evaluation of Bt rice on BPH and PWS should be conducted before commercialization. In the current study, two experiments were performed to assess the potential ecological effects of Bt rice on BPH and PWS: (1) a tritrophic experiment to evaluate the transmission of Cry1Ac, Cry2Aa and Cry1Ca protein in the food chain; and (2) binding assays of Cry1Ac, Cry2Aa and Cry1Ca to midgut brush border membrane proteins from BPH and PWS. Trace amounts of the three Cry proteins were detected in BPH feeding on Bt rice cultivars, but only Cry1Ac and Cry2Aa proteins could be transferred to PWS through feeding on BPH. In vitro binding of biotinylated Cry proteins and competition assays in midgut protein vesicles showed weak binding, and ligand blot analysis confirmed the binding specificity. Thus, we inferred that the tested Bt rice varieties have negligible effect...Continue Reading

References

Apr 13, 2000·Annual Review of Entomology·P C Matteson
Sep 28, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hao ChenQifa Zhang
Apr 7, 2007·Transgenic Research·Natalie FerryAngharad M R Gatehouse
Jun 2, 2007·Environmental Entomology·Fang-Fang LiXue-Xin Chen
Feb 9, 2008·Nature Biotechnology·Jörg RomeisJeffrey D Wolt
Sep 28, 2010·Annual Review of Entomology·Mao ChenGong-yin Ye

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Methods Mentioned

BETA
transgenic
ELISA
enzyme-linked immunosorbent assay
Protein Assay

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