Transgenic drak2 overexpression in mice leads to increased T cell apoptosis and compromised memory T cell development

The Journal of Biological Chemistry
Jianning MaoJiangping Wu

Abstract

Drak2 is a death-associated protein family serine-threonine kinase. Its expression and roles in the immune system were investigated in this study. According to in situ hybridization, Drak2 expression was ubiquitous at the mid-gestation stage in embryos, followed by more focal expression in various organs in the perinatal period and adulthood, notably in the thymus, spleen, lymph nodes, cerebellum, suprachiasmatic nuclei, pituitary, olfactory lobes, adrenal medulla, stomach, skin, and testes. Drak2 transgenic (Tg) mice were generated using the human beta-actin promoter. These Tg mice showed normal T cell versus B cell and CD4 versus CD8 populations in the spleen, but their spleen weight cellularity was lower in comparison with wild type mice. After TCR activation, the proliferation response in Drak2 Tg T cells was normal, although their interleukin (IL)-2 and IL-4 but not interferon-gamma production was augmented. Activated Drak2 Tg T cells demonstrated significantly enhanced apoptosis in the presence of exogenous IL-2. At the molecular level, Drak2 Tg T cells manifested a lower increase of anti-apoptotic factors during activation; such a change probably rendered the cells vulnerable to subsequent IL-2 insults. The compromised a...Continue Reading

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Citations

Jun 25, 2010·Molecular Biology of the Cell·Dagmar Neubueser, David R Hipfner
Nov 5, 2013·Apoptosis : an International Journal on Programmed Cell Death·Ming-Zong Lai, Ruey-Hwa Chen
May 29, 2007·Microbial Pathogenesis·James V RogersCarol L K Sabourin
Sep 9, 2008·Journal of Cellular Biochemistry·Jianning MaoJiangping Wu
Jan 9, 2015·International Immunology·Benjamin A EdwardsMaureen A McGargill
Jul 30, 2009·British Journal of Cancer·G A DohertyD J Fitzgerald
Oct 30, 2007·Autoimmunity·Chris S SchaumburgThomas E Lane
Jun 24, 2008·Autoimmunity·Ahmed Al-QahtaniPaolo Casali
Nov 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Stephanie J RamosCraig M Walsh
Apr 4, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Jianning MaoJiangping Wu
Jun 23, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Martina GatzkaCraig M Walsh
Jul 25, 2019·International Journal of Molecular Sciences·Praveen ManivannanKrishnamurthy Malathi
Nov 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maureen A McGargillStephen M Hedrick
Feb 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Ya-Ting ChuangMing-Zong Lai

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Apoptosis

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