Transgenic epigenetics: using transgenic organisms to examine epigenetic phenomena.

Genetics Research International
Lori A McEachern

Abstract

Non-model organisms are generally more difficult and/or time consuming to work with than model organisms. In addition, epigenetic analysis of model organisms is facilitated by well-established protocols, and commercially-available reagents and kits that may not be available for, or previously tested on, non-model organisms. Given the evolutionary conservation and widespread nature of many epigenetic mechanisms, a powerful method to analyze epigenetic phenomena from non-model organisms would be to use transgenic model organisms containing an epigenetic region of interest from the non-model. Interestingly, while transgenic Drosophila and mice have provided significant insight into the molecular mechanisms and evolutionary conservation of the epigenetic processes that target epigenetic control regions in other model organisms, this method has so far been under-exploited for non-model organism epigenetic analysis. This paper details several experiments that have examined the epigenetic processes of genomic imprinting and paramutation, by transferring an epigenetic control region from one model organism to another. These cross-species experiments demonstrate that valuable insight into both the molecular mechanisms and evolutionary c...Continue Reading

References

May 9, 1991·Nature·M S BartolomeiS M Tilghman
Feb 22, 1991·Cell·T M DeChiaraA Efstratiadis
Aug 27, 1981·Nature·Y GruenbaumA Razin
Sep 1, 1995·Genes & Development·P A LeightonS M Tilghman
Apr 1, 1995·Nature Genetics·K D TremblayM S Bartolomei
Sep 8, 1994·Neuroreport·O Pascalis, S de Schonen
Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·T MooreW Reik
Jan 13, 1998·Nature Genetics·D GarrickE Whitelaw
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·F LykoR Paro
May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·B H RamsahoyeR Jaenisch
Aug 25, 2000·Molecular and Cellular Biology·G WangP B Singh
Dec 16, 2000·Nature·F LykoR Jaenisch
Apr 11, 2001·Genetica·V Lloyd
May 16, 2001·Genomics·M J CranstonM S Bartolomei
Jul 4, 2001·The EMBO Journal·S EdenH Cedar
Jul 5, 2001·Molecular and Cellular Biology·F Sleutels, D P Barlow
Jan 10, 2002·Genes & Development·Adrian Bird
Jan 11, 2002·Developmental Biology·T R HainesP J Ainsworth
Feb 21, 2002·Human Molecular Genetics·Beverly K JonesShirley M Tilghman
Jan 1, 2004·Nature Genetics·Christopher J BeanWilliam G Kelly
Feb 14, 2004·Human Molecular Genetics·Boris KantorAharon Razin
Apr 2, 2004·Insect Molecular Biology·L M FieldG Prantera
May 18, 2004·Current Opinion in Cell Biology·Shiv I S Grewal, Judd C Rice
Jun 24, 2004·Nature Reviews. Genetics·Vicki L Chandler, Maike Stam

❮ Previous
Next ❯

Citations

Jun 26, 2012·Molecular Genetics and Genomics : MGG·Lori A McEachern, Vett K Lloyd

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
histone acetylation
transfection
dissection

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.