Transgenic expression in pig hearts of both human decay-accelerating factor and human membrane cofactor protein does not provide an additional benefit to that of human decay-accelerating factor alone in pig-to-baboon xenotransplantation

Transplantation
Rafael MañezHenk-Jan Schuurman

Abstract

This study investigated whether the coexpression of human decay-accelerating factor (hDAF) and human membrane cofactor protein (hMCP) on porcine organs provides an additional benefit to that of hDAF alone to prevent rejection. Heterotopic heart xenotransplantation was performed in baboons with either hDAF (n=5) or hDAF/hMCP (n=5) transgenic pig organs. The only immunosuppression given was GAS914 (a soluble Gal [alpha1-3] Gal polymer) and cyclosporine A. With the exception of one hDAF organ that failed from a left atrium thrombosis, all xenografts developed acute humoral xenograft rejection. Acute humor xenograft rejection occurred at a median time of 152 hr in hDAF hearts and 162 hr in hDAF/hMCP organs. Recipients of hDAF or hDAF/hMCP hearts did not differ in their patterns of serum antiporcine antibodies or in plasma levels of the soluble terminal complement complex sC5b-9. It is concluded that in this pig-to-baboon heterotopic heart transplant, model expression of hDAF/hMCP does not provide an additional benefit in prevention of rejection to that of hDAF alone.

References

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Citations

Jan 30, 2007·Reproduction, Fertility, and Development·Gábor VajtaZoltán Macháty
Dec 16, 2004·Xenotransplantation·Reto M Baertschiger, Leo H Buhler
Sep 1, 2015·International Journal of Surgery·Muhammad M MohiuddinChristopher G A McGregor
Aug 8, 2014·Xenotransplantation·Guerard W ByrneChristopher G A McGregor
Apr 5, 2014·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·L BurdorfR N Pierson
Feb 1, 2007·Current Opinion in Organ Transplantation·Peter J CowanAnthony Jf dʼApice
Sep 20, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Vasile PavlovPeter S Heeger

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