Transgenic expression of an unedited mitochondrial orfB gene product from wild abortive (WA) cytoplasm of rice (Oryza sativa L.) generates male sterility in fertile rice lines

Planta
Anirban ChakrabortySoumitra K Sen

Abstract

Over-expression of the unedited mitochondrial orfB gene product generates male sterility in fertile indica rice lines in a dose-dependent manner. Cytoplasmic male sterility (CMS) and nuclear-controlled fertility restoration are widespread developmental features in plant reproductive systems. In self-pollinated crop plants, these processes often provide useful tools to exploit hybrid vigour. The wild abortive CMS has been employed in the majority of the "three-line" hybrid rice production since 1970s. In the present study, we provide experimental evidence for a positive functional relationship between the 1.1-kb unedited orfB gene transcript, and its translated product in the mitochondria with male sterility. The generation of the 1.1-kb unedited orfB gene transcripts increased during flowering, resulting in low ATP synthase activity in sterile plants. Following insertion of the unedited orfB gene into the genome of male-fertile plants, the plants became male sterile in a dose-dependent manner with concomitant reduction of ATPase activity of F1F0-ATP synthase (complex V). Fertility of the transgenic lines and normal activity of ATP synthase were restored by down-regulation of the unedited orfB gene expression through RNAi-mediat...Continue Reading

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Citations

Apr 4, 2017·Journal of Experimental Botany·James D StoneHelena Štorchová
Nov 17, 2017·International Journal of Molecular Sciences·Helena Štorchová
Aug 10, 2017·Molecular Biotechnology·Pawan ShuklaPulugurtha Bharadwaja Kirti
Sep 24, 2018·Molecular Biotechnology·Wei Tang, Caroline Luo
Jun 14, 2020·Mitochondrion·Marco ZancaniGiovanna Lippe

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