Transgenic expression of enhanced green fluorescent protein enables direct visualization for physiological studies of vasopressin neurons and isolated nerve terminals of the rat

Endocrinology
Yoichi UetaDavid Murphy

Abstract

We have generated transgenic rats expressing an arginine vasopressin (AVP)-enhanced green fluorescent protein (eGFP) fusion gene. The expression of the eGFP gene and strong fluorescence were observed in the supraoptic nucleus (SON), the paraventricular nucleus (PVN), and the suprachiasmatic nucleus (SCN) in transgenic rats. The hypothalamo-neurohypophyseal tract, isolated SON neurons, and isolated axon terminals in the neurohypophysis also showed robust eGFP fluorescence. Water deprivation for 2 d increased the fluorescence of the eGFP in the SON and the PVN but not the SCN. The whole-cell patch-clamp technique was then used to record the electrical activities specifically identifying eGFP-expressing SON, PVN, and SCN AVP neurons in in vitro brain slice preparations. The AVP-eGFP transgenic rats are a unique new tool with which to study the physiological role of AVP-secreting neurons in the central nervous system and the dynamics of the regulation of AVP secretion in the living neurons and their axon terminals.

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Citations

Jan 13, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Juhee HaamJeffrey G Tasker
Jan 11, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Evgeniy S PotapenkoJavier E Stern
Dec 17, 2010·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Aleksander R ZampronioQuentin J Pittman
Nov 15, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Shi DiJeffrey G Tasker
May 6, 2010·The Journal of Physiology·Toyoaki OhbuchiYoichi Ueta
Jul 27, 2014·The Journal of Physiology·Linda A MortonJeffrey G Tasker
Feb 4, 2010·The Journal of Physiology·Ion R PopescuJeffrey G Tasker
Dec 7, 2010·Neuroscience Letters·Olivia MonteiroMike Ludwig
Sep 6, 2015·The European Journal of Neuroscience·Tomoko YoshikawaKen-Ichi Honma
Jul 16, 2010·CNS Neuroscience & Therapeutics·Cedric VieroGovindan Dayanithi
Mar 11, 2011·Annals of the New York Academy of Sciences·Douglas W WackerMike Ludwig
Dec 15, 2012·The Journal of Comparative Neurology·Benjamin D RoodGeert J De Vries
Mar 28, 2012·Journal of Neuroendocrinology·D MurphyN Japundzic-Zigon
Dec 15, 2010·The European Journal of Neuroscience·Jaclyn I Wamsteeker, Jaideep S Bains
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Mar 6, 2012·Cell Calcium·Govindan DayanithiEmil C Toescu
Feb 22, 2012·Cell Calcium·José R LemosGovindan Dayanithi

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