Transgenic introduction of androgen receptor into estrogen-receptor-, progesterone-receptor-, and androgen-receptor-negative breast cancer cells renders them responsive to hormonal manipulation

American Journal of Surgery
Jennifer R GarreauSuellen Pommier

Abstract

Estrogen-receptor (ER)-, progesterone-receptor (PR)-, and androgen-receptor (AR)-negative breast cancer cells are unaffected by treatment with dehydroepiandrosterone-sulfate (DHEAS) and an aromatase inhibitor (AI). We hypothesized that cell growth would be inhibited with DHEAS/AI treatment after successful transfection of an AR expression vector. ER/PR/AR-negative breast cancer cells were transfected with an AR expression vector and treated with DHEAS/AI for 2 days. Growth inhibition of these cells was compared with that of transfected cells treated with only AI or with nontransfected cells treated with DHEAS/AI. Mann-Whitney U test was used to determine statistical significance. Cell death rates of 53.5% (P = .001) and 40.1% (P = .006) were seen in transfected cells treated with DHEAS/AI compared with controls for days 1 and 2, respectively. Nontransfected cells were unaffected by treatment. ER/PR/AR-negative cells transfected with AR were killed by DHEAS/AI treatment, providing evidence that AR is responsible for this effect. This provides the first AR-targeted hormonal therapy for ER breast cancer.

References

Feb 28, 2002·Journal of Cancer Research and Clinical Oncology·Magdalena BryśWanda M Krajewska
Sep 2, 2004·Journal of the National Cancer Institute·Catherine SchairerSusan S Devesa

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Citations

Apr 11, 2013·Breast Cancer Research and Treatment·C CampagnoliF Berrino
May 10, 2011·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Laura C CollinsRulla M Tamimi
Dec 6, 2008·Folia Histochemica Et Cytobiologica·Anil K AgrawalEwa Nienartowicz
Jul 1, 2012·Drug Discovery Today. Disease Mechanisms·Domenico IacopettaSuzanne Aw Fuqua
Apr 26, 2018·Endocrine-related Cancer·Erin E SwinsteadGordon L Hager
Oct 12, 2018·Endocrinology·Thu H Truong, Carol A Lange
Oct 11, 2007·Menopause : the Journal of the North American Menopause Society·Leon Speroff
Mar 12, 2009·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Ana M Gonzalez-AnguloBryan T Hennessy

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