Transgenic labeling of parvalbumin-expressing neurons with tdTomato

Neuroscience
T KaiserGuoping Feng

Abstract

Parvalbumin (PVALB)-expressing fast-spiking interneurons subserve important roles in many brain regions by modulating circuit function and dysfunction of these neurons is strongly implicated in neuropsychiatric disorders including schizophrenia and autism. To facilitate the study of PVALB neuron function we need to be able to identify PVALB neurons in vivo. We have generated a bacterial artificial chromosome (BAC) transgenic mouse line expressing the red fluorophore tdTomato under the control of endogenous regulatory elements of the Pvalb gene locus (JAX # 027395). We show that the tdTomato transgene is faithfully expressed relative to endogenous PVALB expression throughout the brain. Furthermore, targeted patch clamp recordings confirm that the labeled populations in neocortex, striatum, and hippocampus are fast-spiking interneurons based on intrinsic properties. This new transgenic mouse line provides a useful tool to study PVALB neuron function in the normal brain as well as in mouse models of psychiatric disease.

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Citations

Feb 9, 2016·Neuroscience·Joshua A Gordon, Kafui Dzirasa
Jul 15, 2017·Journal of the American Society of Nephrology : JASN·James A McCormick, David H Ellison
Jul 24, 2020·Nature·Yinqing LiGuoping Feng
Jul 30, 2020·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Jacob B RudenChristine Konradi
Mar 19, 2019·The European Journal of Neuroscience·Federica FiliceBeat Schwaller
Sep 22, 2020·Brain Communications·Daniel Enterría-MoralesXavier d'Anglemont de Tassigny
Jul 2, 2021·Molecular and Cellular Neurosciences·Cheryl BrandenburgGene J Blatt

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