Transgenic lambda medaka as a new model for germ cell mutagenesis

Environmental and Molecular Mutagenesis
Richard N WinnMichelle B Norris

Abstract

To address the need for improved approaches to study mutations transmitted to progeny from mutagen-exposed parents, we evaluated lambda transgenic medaka, a small fish that carries the cII mutation target gene, as a new model for germ cell mutagenesis. Mutations in the cII gene in progeny derived from ethyl-nitrosourea (ENU)-exposed males were readily detected. Frequencies of mutant offspring, proportions of mosaic or whole body mutant offspring, and mutational spectra differed according to germ cell stage exposed to ENU. Postmeiotic germ cells (spermatozoa/late spermatids) generated a higher frequency of mutant offspring (11%) compared to premeiotic germ cells (3.5%). Individuals with cII mutant frequencies (MF) elevated more than threefold above the spontaneous MF (3 x 10(-5)) in the range of 10(-4) to 10(-3) were mosaic mutant offspring, whereas those with MFs approaching 1 x 10(-2) were whole body mutant offspring. Mosaic mutant offspring comprised the majority of mutant offspring derived from postmeiotic germ cells, and unexpectedly, from spermatogonial stem cells. Mutational spectra comprised of two different mutations, but at identical sites were unusual and characteristic of delayed mutations, in which fixation of a sec...Continue Reading

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Jun 3, 2009·Nature Reviews. Genetics·Norman Arnheim, Peter Calabrese
May 2, 2014·Mutation Research. Genetic Toxicology and Environmental Mutagenesis·Alaa El-Din Hamid SayedHiroshi Mitani
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Dec 15, 2015·Aquatic Toxicology·Melissa ChernickDavid E Hinton
Feb 15, 2017·Chemistry : a European Journal·Steve UllmannBerthold Kersting

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