PMID: 9163338Apr 15, 1997Paper

Transgenic mice overexpressing ornithine and S-adenosylmethionine decarboxylases maintain a physiological polyamine homoeostasis in their tissues

The Biochemical Journal
R HeljasvaaraA Pajunen

Abstract

Recent work has shown that transgenic mice overexpressing human ornithine decarboxylase display no marked changes in the tissue concentrations of spermidine or spermine in spite of a dramatic increase in putrescine levels. In the tissues of transgenic mice carrying the human spermidine synthase gene and in those of hybrid mice overexpressing both ornithine decarboxylase and spermidine synthase, spermidine and spermine levels remain within normal limits. To test whether the amount of the propylamine group donor, decarboxylated S-adenosylmethionine, limits the conversion of putrescine into the higher polyamines, we have produced transgenic mouse lines harbouring the rat S-adenosylmethionine decarboxylase gene in their genome. However, neither these mice nor the hybrid mice overexpressing both ornithine decarboxylase and S-adenosylmethionine decarboxylase displayed significant changes in their spermidine and spermine tissue levels. To study the mechanism by which cells maintain the constancy of the polyamine concentrations, we have determined the metabolic flux of polyamines in transgenic primary fibroblasts using pulse labelling. The results indicate that the polyamine flow is faster in transgenic primary fibroblasts than in non-...Continue Reading

Citations

Jun 11, 2002·Biochemical and Biophysical Research Communications·Carmen M BastidaRafael Peñafiel
Sep 11, 2001·The American Journal of Pathology·N AhmadH Mukhtar
Feb 26, 2013·Expert Reviews in Molecular Medicine·Shannon L NowotarskiRobert A Casero
Jun 15, 2007·Critical Reviews in Clinical Laboratory Sciences·Nikolaus Seiler, Francis Raul
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