PMID: 9557658Apr 29, 1998Paper

Transgenic mice secreting coronavirus neutralizing antibodies into the milk

Journal of Virology
I SolaLuis Enjuanes

Abstract

Ten lines of transgenic mice secreting transmissible gastroenteritis coronavirus (TGEV) neutralizing recombinant monoclonal antibodies (rMAbs) into the milk were generated. The rMAb light- and heavy-chain genes were assembled by fusing the genes encoding the variable modules of the murine MAb 6A.C3, which binds an interspecies conserved coronavirus epitope essential for virus infectivity, and a constant module from a porcine myeloma with the immunoglobulin A (IgA) isotype. The chimeric antibody led to dimer formation in the presence of J chain. The neutralization specific activity of the recombinant antibody produced in transiently or stably transformed cells was 50-fold higher than that of a monomeric rMAb with the IgG1 isotype and an identical binding site. This rMAb had titers of up to 10(4) by radioimmunoassay (RIA) and neutralized virus infectivity up to 10(4)-fold. Of 23 transgenic mice, 17 integrated both light and heavy chains, and at least 10 of them transmitted both genes to the progeny, leading to 100% of animals secreting functional TGEV neutralizing antibody during lactation. Selected mice produced milk with TGEV-specific antibody titers higher than 10(6) as determined by RIA, neutralized virus infectivity by 10(6)...Continue Reading

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Citations

Nov 10, 2004·Proceedings of the National Academy of Sciences of the United States of America·Etsuro OnoToshimitsu Uede
Feb 13, 2016·The Journal of Dairy Research·C Bruce A WhitelawChris Proudfoot
Oct 21, 2017·Reproduction in Domestic Animals = Zuchthygiene·I Lamas-ToranzoP Bermejo-Álvarez
Mar 29, 2014·BMC Veterinary Research·Weidong ChaiMichael Burwinkel

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