Transgenic mice that accept Luciferase- or GFP-expressing syngeneic tumor cells at high efficiencies

Genes to Cells : Devoted to Molecular & Cellular Mechanisms
Naoki AoyamaMakoto Mark Taketo

Abstract

Jellyfish green fluorescent protein (GFP) and firefly luciferase can serve as versatile tracking markers for identification and quantification of transplanted cancer cells in vivo. However, immune reactions against these markers can hamper the formation of syngraft tumors and metastasis that follows. Here, we report two transgenic (Tg) mouse lines that express nonfunctional mutant marker proteins, namely modified firefly luciferase (Luc2) or enhanced GFP (EGFP). These mice, named as Tg-mLuc2 and Tg-mEGFP, turned out to be immunologically tolerant to the respective tracking markers and thus efficiently accepted syngeneic cancer cells expressing the active forms of the markers. We then injected intrarectally the F1 hybrid Tg mice (BALB/c × C57BL/6J) with Colon-26 (C26) colon cancer cells that originated from a BALB/c mouse. Even when C26 cells expressed active Luc2 or EGFP, they formed primary tumors in the Tg mice with only 104 cells per mouse compared with more than 106 cells required in the nontransgenic BALB/c hosts. Furthermore, we detected metastatic foci of C26 cells in the liver and lungs of the Tg mice by tracking the specific reporter activities. These results show the usefulness of the Tg mouse lines as recipients for ...Continue Reading

References

Jun 1, 1992·Surgical Oncology·H KashtanS Gallinger
Jan 1, 1989·Cancer Chemotherapy and Pharmacology·M M Tomayko, C P Reynolds
May 5, 1997·FEBS Letters·M OkabeY Nishimune
Jul 25, 1997·The Journal of Biological Chemistry·J F ThompsonB R Branchini
Oct 6, 1998·Annual Review of Biochemistry·R Y Tsien
May 13, 1999·Immunological Reviews·C AmadouK F Lindahl
Mar 30, 2006·Transplantation·William H KitchensJoren C Madsen
Feb 22, 2008·Gene Therapy·W G H HanM H M Wauben
Jun 2, 2009·Transgenic Research·Krisztián KvellZsuzsanna Bosze
Aug 19, 2009·Surgical Endoscopy·Melissa DoniganCheryl H Baker
Jul 29, 2010·Physiological Reviews·Dmitriy M ChudakovKonstantin A Lukyanov
Dec 29, 2010·Cellular and Molecular Life Sciences : CMLS·Saman Hosseinkhani
Jan 22, 2011·Cancer Cell·Masahiro SonoshitaMakoto Mark Taketo
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jumpei KondoMasahiro Inoue
Nov 10, 2013·Nature Medicine·Daniela F Quail, Johanna A Joyce
Nov 16, 2013·Nature Protocols·Hiroyuki Miyoshi, Thaddeus S Stappenbeck
Nov 23, 2013·PloS One·Hiroyuki KishimotoRobert M Hoffman
Mar 25, 2014·Current Opinion in Genetics & Development·Sadna BudhuTaha Merghoub
Dec 24, 2014·Nature Reviews. Immunology·Linh T Nguyen, Pamela S Ohashi
Jan 24, 2015·Nature Reviews. Immunology·Takanori KitamuraJeffrey W Pollard
Apr 11, 2015·Cancer Cell·Suzanne L TopalianDrew M Pardoll
Jul 21, 2015·Nature Cell Biology·Wei-Yu LuStuart J Forbes
Feb 26, 2016·Annals of Oncology : Official Journal of the European Society for Medical Oncology·M F SanmamedH Kohrt
Jan 18, 2017·Proceedings of the National Academy of Sciences of the United States of America·Kenji ChamotoTasuku Honjo
May 4, 2017·Journal of Immunological Methods·Roman DovhyiLarysa Skivka
May 16, 2017·Trends in Biotechnology·Laura MezzanotteClemens W G M Löwik
May 17, 2017·Oncoimmunology·Jan Willem KleinovinkMarieke F Fransen

❮ Previous
Next ❯

Citations

May 31, 2020·Communications Biology·Kaspar BresserTon N Schumacher
Sep 15, 2020·FASEB BioAdvances·Natasha L FriendAndrew C W Zannettino
Jul 10, 2020·Cancer Research·Manuel ValienteFrank Winkler
Jul 3, 2021·International Journal of Molecular Sciences·Cuihua LiuBing Wang

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.