Transgenic mice with mammary gland targeted expression of human cortactin do not develop (pre-malignant) breast tumors: studies in MMTV-cortactin and MMTV-cortactin/-cyclin D1 bitransgenic mice

BMC Cancer
Agnes G S H van RossumE Schuuring

Abstract

In human breast cancers, amplification of chromosome 11q13 correlates with lymph node metastasis and increased mortality. To date, two genes located within this amplicon, CCND1 and EMS1, were considered to act as oncogenes, because overexpression of both proteins, respectively cyclin D1 and cortactin, correlated well with 11q13 amplification. Cyclin D1 is involved in cell cycle regulation and the F-actin-binding protein cortactin in cytoskeletal dynamics and cell migration. To study the role of cortactin in mammary gland tumorigenesis, we examined mouse mammary tumor virus (MMTV)-cortactin transgenic mice and MMTV-cortactin/-MMTV-cyclin D1 bitransgenic mice. MMTV-cortactin transgenic mice were generated and intercrossed with previously described MMTV-cyclin D1 transgenic mice. Immunohistochemical, Northern and Western blot analyses were performed to study the expression of human transgene cortactin during mammary gland development and in mammary tumors. For tumor incidence studies, forced-bred, multiparous mice were used to enhance transgene expression in the mammary gland. Microscopical examination was performed using haematoxylin and eosin staining. Mammary gland tumors arose stochastically (incidence 21%) with a mean age of ...Continue Reading

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Citations

Jan 22, 2010·The Journal of Biological Chemistry·Bharat JoshiIvan R Nabi
Oct 1, 2009·Future Medicinal Chemistry·Teresa T BonelloPeter W Gunning
Jan 28, 2012·Journal of Proteomics·Pablo R GrigeraJay W Fox
Nov 28, 2012·The Journal of Pathology·Christelle SeigneChang Xian Zhang
Mar 10, 2012·Journal of the Korean Surgical Society·Seok Youn LeeSoo Cheon Chae
Jan 25, 2011·Cell Adhesion & Migration·Kellye C KirkbrideAlissa M Weaver

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Methods Mentioned

BETA
transgenic
Protein assay

Software Mentioned

SPSS

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