Transgenic mice with osteoblast-targeted insulin-like growth factor-I show increased bone remodeling

Bone
Jin JiangB E Kream

Abstract

To determine the effects of locally-expressed insulin-like growth factor (IGF-I) on bone remodeling, a transgene was produced in which murine IGF-I cDNA was cloned downstream of a gene fragment comprising 3.6 kb of 5' upstream regulatory sequence and most of the first intron of the rat Col1a1 gene. The construct was expressed at the mRNA and protein level in transfected osteoblasts. Five lines of transgenic mice were generated by embryo microinjection. Transgene mRNA levels were highest in calvaria, long bone and tendon, and lower in skin. Serum IGF-I and body weight were increased in males and females only in the highest expressing line. Histomorphometry showed that transgenic calvaria were wider and had greater marrow area and bone area. Transgenic calvaria had increased osteoclast number per bone surface. Percent collagen synthesis and cell replication were increased in transgenic calvaria. Femur length, cortical width and cross-sectional area were increased in transgenic femurs of the highest expressing line, while femoral trabecular bone volume was little affected. Thus, broad overexpression of IGF-I in cells of the osteoblast lineage increased indices of bone formation and resorption.

References

Jan 1, 1989·The Journal of Clinical Investigation·E CanalisT L McCarthy
Jul 1, 1972·Developmental Biology·R F Diegelmann, B Peterkofsky
Oct 1, 1980·The Journal of Clinical Investigation·E Canalis
Aug 6, 1996·Proceedings of the National Academy of Sciences of the United States of America·K ReissP Anversa
Jun 9, 1999·Proceedings of the National Academy of Sciences of the United States of America·K SjögrenC Ohlsson
Jun 23, 1999·Proceedings of the National Academy of Sciences of the United States of America·S YakarD LeRoith
Jul 28, 1999·Molecular Reproduction and Development·M K DyckF Pothier
Sep 13, 2000·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·D Le Roith
May 9, 2001·The Journal of Clinical Investigation·Y ImanishiA Arnold
Sep 18, 2002·The Journal of Clinical Investigation·Shoshana YakarDerek LeRoith

❮ Previous
Next ❯

Citations

Apr 1, 2008·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·D D Bikle
Jul 5, 2007·Histochemistry and Cell Biology·Lindsay M Martin, Laura R McCabe
Feb 28, 2008·Journal of Bone and Mineral Metabolism·Jian-min LiuJia-lun Chen
May 1, 2012·Current Osteoporosis Reports·Subburaman Mohan, Chandrasekhar Kesavan
Jan 15, 2014·BoneKEy Reports·Anyonya R Guntur, Clifford J Rosen
Jul 11, 2009·Endocrine Reviews·Claes OhlssonJohan Svensson
Feb 12, 2009·Journal of Cell Science·Aditi Mukherjee, Peter Rotwein
Mar 30, 2011·PloS One·Hayden-William CourtlandShoshana Yakar
Feb 19, 2014·Archives of Oral Biology·Hiroyuki TakenouchiNoriyoshi Shimizu
Sep 27, 2013·Journal of Molecular Medicine : Official Organ of the Gesellschaft Deutscher Naturforscher Und Ärzte·Janet L Crane, Xu Cao
Jul 27, 2014·Materials Science & Engineering. C, Materials for Biological Applications·Venkata P Mantripragada, Ambalangodage C Jayasuriya
May 6, 2009·Climacteric : the Journal of the International Menopause Society·C RuizA Cano
Jun 12, 2012·Endocrinology and Metabolism Clinics of North America·Shoshana Yakar, Martin L Adamo
Nov 8, 2011·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Tomasz GredesChristiane Kunert-Keil
Oct 22, 2011·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Sridhar MuthusamiSrinivasan Narasimhan
Feb 24, 2007·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Aditi Mukherjee, Peter Rotwein
Apr 26, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Alice Fiona Ford-HutchinsonFrank Robert Jirik
Aug 31, 2006·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·John L FowlkesCharles K Lumpkin
May 16, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Lorenz C HofbauerHarald Dobnig
Dec 19, 2007·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Sharon J TannerCheryl A Conover
Jul 22, 2009·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J Christopher FrittonShoshana Yakar
Mar 5, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Sebastien ElisShoshana Yakar
Sep 27, 2015·Molecular and Cellular Endocrinology·Richard C Lindsey, Subburaman Mohan
Oct 4, 2015·Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society·Shoshana Yakar, Olle Isaksson
May 20, 2008·Bone·Sol Epstein, Derek LeRoith
Nov 11, 2008·Developmental Biology·S J ChoiT C Hart
Oct 4, 2016·PloS One·Melissa N van TokLeonie M van Duivenvoorde
Mar 30, 2011·The International Journal of Lower Extremity Wounds·Robert BlakytnyEdward B Jude
Oct 6, 2017·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Faming TianDaniel D Bikle
Sep 29, 2017·Osteoporosis International : a Journal Established As Result of Cooperation Between the European Foundation for Osteoporosis and the National Osteoporosis Foundation of the USA·C LavetP Ammann
Apr 8, 2018·Journal of Molecular Endocrinology·Shoshana YakarClifford J Rosen
Mar 28, 2018·Journal of Molecular Endocrinology·Richard C LindseySubburaman Mohan

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
Mary BouxseinW G Beamer
Growth Hormone & IGF Research : Official Journal of the Growth Hormone Research Society and the International IGF Research Society
O G IsakssonJ O Jansson
© 2022 Meta ULC. All rights reserved