Transgenic microRNA inhibition with spatiotemporal specificity in intact organisms.

Nature Methods
Carlos M LoyaTudor A Fulga

Abstract

MicroRNAs are important regulators of gene expression, yet the functional outputs of most microRNA-target interactions remain elusive. Here we introduce the Drosophila melanogaster microRNA sponge (miR-SP) as a powerful transgenic technology to dissect the function of every microRNA with precise spatiotemporal resolution. miR-SPs can be used to characterize tissue-specific microRNA loss-of-function phenotypes, define the spatial regulation of their effectors and uncover interactions between microRNAs and other genes. Using themiR-SP system, we identified an essential role of the conserved microRNA miR-8, in neuromuscular junction formation. Tissue-specific silencing revealed that postsynaptic activity of miR-8 is important for normal neuromuscular junction morphogenesis. Given that miR-SPs rely on a bipartite modular expression system, they could be used to elucidate the endogenous function of microRNAs in any species in which conditional expression can be achieved.

References

Mar 17, 2004·PLoS Biology·György HutvágnerPhillip D Zamore
Mar 24, 2004·Trends in Neurosciences·Haig Keshishian, You-Seung Kim
Sep 24, 2004·RNA·Marc RehmsmeierRobert Giegerich
Dec 7, 2005·Proceedings of the National Academy of Sciences of the United States of America·A Aziz AboobakerEric C Lai
Nov 23, 2006·Nature Reviews. Neuroscience·Kenneth S Kosik
Jan 19, 2007·Current Opinion in Neurobiology·Catherine A Collins, Aaron DiAntonio
May 18, 2007·Annual Review of Cell and Developmental Biology·Natascha Bushati, Stephen M Cohen
Jul 7, 2007·Molecular Cell·Andrew GrimsonDavid P Bartel
Aug 19, 2007·Nature Methods·Margaret S EbertPhillip A Sharp
Nov 9, 2007·Proceedings of the National Academy of Sciences of the United States of America·Serena J SilverEric C Lai
Jan 15, 2008·Cell·Ana EulalioElisa Izaurralde
Jan 30, 2008·PLoS Genetics·Hideya Kawaji, Yoshihide Hayashizaki
Apr 16, 2008·Journal of Neurochemistry·Giovanni LugliNeil R Smalheiser
May 17, 2008·Development, Growth & Differentiation·Kazuhide Asakawa, Koichi Kawakami
May 30, 2008·Current Opinion in Neurobiology·Frauke Drees, Frank B Gertler
Jul 22, 2008·Methods in Molecular Biology·David A Elliott, Andrea H Brand
Sep 10, 2008·Proceedings of the National Academy of Sciences of the United States of America·Patrick SternRichard O Hynes
Jul 18, 2009·Nature Reviews. Genetics·Brian D Brown, Luigi Naldini

❮ Previous
Next ❯

Citations

Mar 15, 2011·Biotechnology Letters·Marlene ReichelAnthony A Millar
Aug 6, 2011·Pharmaceutical Research·Ailbhe M McDermottMichael J Kerin
Jun 19, 2010·Journal of Cardiovascular Translational Research·Reena V Kartha, Subbaya Subramanian
Sep 27, 2013·Neurotherapeutics : the Journal of the American Society for Experimental NeuroTherapeutics·Miguel A VarelaMatthew J A Wood
May 18, 2013·Trends in Parasitology·Keira J LucasAlexander S Raikhel
Dec 15, 2010·ACS Chemical Biology·Frederic D Sigoillot, Randall W King
Apr 29, 2011·Gene Therapy·J A Broderick, P D Zamore
Jun 12, 2013·Molecular Therapy : the Journal of the American Society of Gene Therapy·Rasmus O BakJacob Giehm Mikkelsen
Feb 18, 2012·Nature·Amaia Lujambio, Scott W Lowe
Mar 23, 2011·Nature Genetics·Susanna ObadSakari Kauppinen
Jun 26, 2012·Nature Neuroscience·Antoine de ChevignyHarold Cremer
Oct 5, 2010·Nature Reviews. Drug Discovery·Ramiro GarzonCarlo M Croce
Jun 27, 2013·Nature Reviews. Molecular Cell Biology·Stefan L Ameres, Phillip D Zamore
Apr 3, 2010·Genes & Development·Carlos M LoyaTudor A Fulga
Mar 17, 2010·Silence·Stefan L Ameres, Ryuya Fukunaga
Sep 22, 2010·RNA·Margaret S Ebert, Phillip A Sharp
Jan 13, 2012·PloS One·Joost KluiverAnke van den Berg
Dec 19, 2013·PloS One·Xiangqian GuoRunsheng Chen
Mar 29, 2013·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Wanhe LiJosh Dubnau
Sep 17, 2011·Journal of Nucleic Acids·Andrius ServaVytaute Starkuviene
Apr 11, 2014·Development·Carlos M LoyaDavid Van Vactor
Nov 26, 2013·Leukemia·S EmmrichM M van den Heuvel-Eibrink
Aug 20, 2014·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Cecilia S LuDavid Van Vactor
Jun 2, 2011·Journal of Aging Research·Andrea FelicianoMatilde E Lleonart
Apr 24, 2014·Ageing Research Reviews·Devika Garg, Stephen M Cohen
May 11, 2015·FEBS Letters·Petr Svoboda
Jan 22, 2015·Proceedings of the National Academy of Sciences of the United States of America·Keira J LucasAlexander S Raikhel
Nov 21, 2012·Insect Biochemistry and Molecular Biology·Keira Lucas, Alexander S Raikhel
Oct 6, 2015·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Rajeswari BanerjiRobert V Skibbens
Oct 8, 2011·Cellular Signalling·Megan Y MurrayDavid J MacEwan

❮ Previous
Next ❯

Datasets Mentioned

BETA
GMR-Gal4

Methods Mentioned

BETA
transgenic
PCR
dissection

Software Mentioned

TargetScanFly
OpenLab
Elements
TargetScan
Zeiss LSM Multi - Time
Zeiss LSM

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.