PMID: 9418047Jan 7, 1998Paper

Transgenic potato tubers accumulate high levels of 1-kestose and nystose: functional identification of a sucrose sucrose 1-fructosyltransferase of artichoke (Cynara scolymus) blossom discs

The Plant Journal : for Cell and Molecular Biology
E M HellwegeA G Heyer

Abstract

By screening a cDNA library of artichoke (Cynara scolymus) blossom discs for fructosyltransferases, we isolated a clone designated Cy21. The deduced amino acid sequence shows homology to acid beta-fructosyl hydrolases and to the sucrose-fructan 6-fructosyltransferase (6-SFT) of barley. Transiently expressed in Nicotiana tabacum protoplasts, the Cy21 gene-product synthesized 1-kestose, indicating that Cy21 codes for a sucrose sucrose 1-fructosyltransferase (1-SST). The enzyme worked at physiologically relevant sucrose concentrations (25 mM sucrose). In the protoplast system, 1-kestose seemed to be the only fructan product of the 1-SST. The enzyme activity was not affected by pyridoxal-HCl, an inhibitor of both the beta-fructosyl hydrolase and the fructosyltransferase activity of invertases. The fructosyltransferase activity of the Cy21 gene-product, however, could be inhibited by Zn2+, Ag+ and Cu2+ ions. In artichoke plants the Cy21 transcript was highly abundant in primary roots and blossom discs. Transgenic potato tubers expressing Cy21 contain high levels of 1-kestose along with nystose and traces of fructosyl-nystose, supporting the conclusion that the Cy21 clone encodes a sucrose sucrose 1-fructosyltransferase.

Citations

Mar 18, 2009·Cellular and Molecular Life Sciences : CMLS·David P LivingstonArnd G Heyer
Feb 7, 2002·Phytochemistry·Horacio G PontisEd Etxeberria
Apr 21, 1999·Current Opinion in Biotechnology·A G HeyerJ Kossmann
May 20, 2003·Current Opinion in Plant Biology·Tita Ritsema, Sjef Smeekens
Sep 22, 1998·Nature Biotechnology·S Smeekens
Sep 22, 1998·Nature Biotechnology·R SévenierA J Koops
Jan 29, 2000·The Plant Journal : for Cell and Molecular Biology·T PeterbauerA Richter
Jan 13, 2000·European Journal of Biochemistry·D K HinchaJ H Crowe
Jan 1, 2003·Journal of Experimental Botany·Andrew J Cairns
Jul 10, 2008·Plant Physiology·Martina Newell-McGloughlin
Apr 30, 2009·Annual Review of Nutrition·Kendal D Hirschi
Jul 13, 2000·Proceedings of the National Academy of Sciences of the United States of America·E M HellwegeA G Heyer
Dec 19, 2006·Plant Biotechnology Journal·Jaye ChalmersGerman Spangenberg
Oct 5, 2006·The Plant Journal : for Cell and Molecular Biology·Tita RitsemaSjef Smeekens
Mar 8, 2013·Carbohydrate Polymers·Jeroen van ArkelIngrid M van der Meer
Apr 17, 2007·Current Opinion in Plant Biology·Anna LytovchenkoAlisdair R Fernie
Jun 11, 1999·Plant Physiology·I Vijn, S Smeekens
Jul 27, 2010·New Biotechnology·Martina Newell McGloughlin

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