PMID: 8977445Dec 1, 1996Paper

Transgenic rabbits expressing human apolipoprotein A-I in the liver

Arteriosclerosis, Thrombosis, and Vascular Biology
N DuvergerP Denèfie

Abstract

Human apolipoprotein A-I (apo A-I) transgenic rabbits were created by use of an 11-kb genomic human apo A-I construct containing a liver-specific promoter. Five independent transgenic lines were obtained in which human apo A-I gene had integrated and was expressed. Plasma levels of human apo A-I ranged from 8 to 100 mg/dL for the founder and up to 175 mg/dL for the progeny. Rabbit apo A-I levels were substantially decreased in the transgenic rabbits. HDL cholesterol (HDL-C) levels were higher in two of the five transgenic rabbit lines than in controls (line 20 versus nontransgenic littermate, HDL-C = 80 +/- 7 versus 37 +/- 6 mg/dL; line 8 versus nontransgenic littermate, HDL-C = 54 +/- 16 versus 35 +/- 6 mg/dL). This resulted in less atherogenic lipoprotein profiles, with very low (VLDL + LDL-C)/HDL-C ratios. HDL size and protein and lipid compositions were similar between transgenic and littermate nontransgenic rabbits. However, a large amount of pre-beta apo A-I-containing lipoproteins was observed in the plasma of the highest human apo A-I expressor. Cell cholesterol efflux was evaluated with the incubation of whole serum from transgenic and control rabbits. Cell cholesterol efflux was highly correlated with HDL cholesterol,...Continue Reading

References

Feb 1, 1978·Journal of Immunogenetics·A L Børresen, T J Kindt
Sep 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·J R PatschS Eisenberg
May 1, 1991·Journal of Biotechnology·M MassoudL M Houdebine
May 1, 1988·Proceedings of the National Academy of Sciences of the United States of America·K L KnightV T Oi
Apr 3, 1989·Biochimica Et Biophysica Acta·M A ClayP J Barter
Mar 1, 1987·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·A La VilleB Lewis
Jan 1, 1986·Methods in Enzymology·A V NicholsT A Musliner
Nov 1, 1986·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·T TsukadaA M Gown
Nov 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·J L BreslowV I Zannis
Jan 1, 1983·Arteriosclerosis : an Official Journal of the American Heart Association, Inc·L M BujaM S Brown
May 29, 1981·Biochemical and Biophysical Research Communications·K ShiraiR L Jackson
Aug 30, 1994·Proceedings of the National Academy of Sciences of the United States of America·J FanJ M Taylor

❮ Previous
Next ❯

Citations

Mar 1, 2000·Biochemical and Biophysical Research Communications·M MacknessA Bouiller
Oct 8, 1999·Clinica Chimica Acta; International Journal of Clinical Chemistry·P Duriez, J C Fruchart
Jul 27, 2002·Theriogenology·Hiroshi MurakamiHiroshi Imai
Sep 3, 2003·Pharmacology & Therapeutics·Jianglin Fan, Teruo Watanabe
Feb 8, 2003·Atherosclerosis. Supplements·Roger S Newton, Brian R Krause
Nov 13, 2008·Cytotechnology·M ArakiT Watanabe
Nov 3, 2005·Nature Clinical Practice. Cardiovascular Medicine·M M ThompsonG W Cockerill
Aug 17, 2000·Current Opinion in Lipidology·J Stocks
Dec 14, 2004·Lipids in Health and Disease·Florence GondretJean-François Hocquette
Aug 14, 2002·American Journal of Pharmacogenomics : Genomics-related Research in Drug Development and Clinical Practice·S C FrancisM K Raizada
Jan 23, 2017·Journal of Dairy Science·Germán G KaiserAdrián A Mutto
Jun 28, 2001·Journal of Atherosclerosis and Thrombosis·J SasakiT Koga
Feb 6, 2016·Cardiovascular Drugs and Therapy·Yanhong GuoY Eugene Chen
Apr 13, 2000·International Journal of Experimental Pathology·G A Ferns, T Y Avades
Oct 20, 2017·Journal of Atherosclerosis and Thrombosis·Jianglin FanJingyan Liang
Feb 20, 2021·Frontiers in Genetics·Jianglin FanY Eugene Chen
Feb 12, 1998·Biochemical and Biophysical Research Communications·G LebuffeP Duriez
Aug 26, 2003·The American Journal of Pathology·Manal ZabalawiMary G Sorci-Thomas

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.