Transgenic rice grains expressing a heterologous ρ-hydroxyphenylpyruvate dioxygenase shift tocopherol synthesis from the γ to the α isoform without increasing absolute tocopherol levels.

Transgenic Research
Gemma FarréChangfu Zhu

Abstract

We generated transgenic rice plants overexpressing Arabidopsis thaliana ρ-hydroxyphenylpyruvate dioxygenase (HPPD), which catalyzes the first committed step in vitamin E biosynthesis. Transgenic grains accumulated marginally higher levels of total tocochromanols than controls, reflecting a small increase in absolute tocotrienol synthesis (but no change in the relative abundance of the α and γ isoforms). In contrast, there was no change in the absolute tocopherol level, but a significant shift from the γ to the α isoform. These data confirm HPPD is not rate limiting, and that increasing flux through the early pathway reveals downstream bottlenecks that act as metabolic tipping points.

References

Aug 17, 1999·Annual Review of Nutrition·M G Traber, H Arai
Nov 25, 2003·The Plant Cell·Alison L Van EenennaamRobert L Last
Mar 27, 2004·Trends in Biotechnology·Imad Ajjawi, David Shintani
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Dec 22, 2009·Current Opinion in Plant Biology·Gemma FarrePaul Christou

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Citations

Mar 4, 2014·Annual Review of Plant Biology·Gemma FarréChangfu Zhu
Nov 2, 2016·Annals of the New York Academy of Sciences·Dieter BlancquaertDominique Van Der Straeten
Oct 30, 2015·Critical Reviews in Food Science and Nutrition·Deep Shikha BirlaPawan K Jaiwal
Dec 16, 2019·Applied Microbiology and Biotechnology·Panhong YuanLong Liu
Jan 21, 2020·Critical Reviews in Food Science and Nutrition·Liyou ZhengQingzhe Jin
Jun 22, 2017·Frontiers in Plant Science·Ling JiangChunyi Zhang

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