PMID: 9193076May 1, 1997Paper

Transgenic rice (Oryza sativa) endosperm expressing daffodil (Narcissus pseudonarcissus) phytoene synthase accumulates phytoene, a key intermediate of provitamin A biosynthesis

The Plant Journal : for Cell and Molecular Biology
P K BurkhardtIngo Potrykus

Abstract

Rice (Oryza sativa L.), the major food staple for more than two billion people, contains neither beta-carotene (provitamin A) nor C40 carotenoid precursors thereof in its endosperm. To improve the nutritional value of rice, genetic engineering was chosen as a means to introduce the ability to make beta-carotene into rice endosperm tissue. Investigation of the biochemical properties of immature rice endosperm using [14C]-labelled substrates revealed the presence of geranyl geranyl diphosphate, the C20 general isoprenoid precursor necessary for C40 carotenoid biosynthesis. Phytoene synthase, which condenses two molecules of geranyl geranyl diphosphate, is the first of four specific enzymes necessary for beta-carotene biosynthesis in plants. Therefore, the Japonica rice model variety Taipei 309 was transformed by microprojectile bombardment with a cDNA coding for phytoene synthase from daffodil (Narcissus pseudonarcissus) under the control of either a constitutive or an endosperm-specific promoter. In transgenic rice plants, the daffodil enzyme is active, as measured by the in vivo accumulation of phytoene in rice endosperm. Thus, it is demonstrated for the first time that it is in principle possible to engineer a critical step in...Continue Reading

Citations

Mar 26, 2009·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·A SinghA K Singh
Jun 17, 2009·Applied Microbiology and Biotechnology·Guan-Nan LiuJian-Guo Jiang
Nov 6, 2009·Molecular Genetics and Genomics : MGG·Neetu ChaudharyParamjit Khurana
Oct 3, 2008·Transgenic Research·Ganesan SunilkumarKeerti S Rathore
Dec 4, 2003·Metabolic Engineering·Monica P RavanelloChristine K Shewmaker
Sep 21, 2000·Plant Science : an International Journal of Experimental Plant Biology·A K Tyagi, A Mohanty
Oct 11, 2003·Biotechnology Advances·C C Giri, G Vijaya Laxmi
Apr 21, 1999·Current Opinion in Biotechnology·J Hirschberg
Oct 25, 2000·Trends in Plant Science·G GiulianoS Dharmapuri
Apr 20, 2001·Current Opinion in Plant Biology·J Hirschberg
Mar 6, 1999·Current Opinion in Plant Biology·T KomariT Kubo
Oct 20, 2005·Journal of Bioscience and Bioengineering·K Yoshida, A Shinmyo
Mar 29, 2005·Nature Biotechnology·Jacqueline A PaineRachel Drake
Dec 22, 1999·The Plant Journal : for Cell and Molecular Biology·C K ShewmakerD Y Ke
Aug 17, 2002·The Plant Journal : for Cell and Molecular Biology·Stanley P KowalskiRobert H Potter
Jun 1, 2004·Nutrition Research Reviews·Paul Christou, Richard M Twyman
May 5, 2006·The Proceedings of the Nutrition Society·C SautterW Gruissem
Sep 11, 2010·Critical Reviews in Food Science and Nutrition·K K Namitha, P S Negi
Feb 8, 2003·DNA and Cell Biology·Nigel J Taylor, Claude M Fauquet
Aug 30, 2008·Journal of Experimental Botany·Maneesha AluruSteve Rodermel
Feb 11, 2009·Journal of Experimental Botany·Masaki FujisawaNorihiko Misawa
Oct 24, 2002·Journal of Pediatric Gastroenterology and Nutrition·Hilary A Perr
Sep 13, 2003·Plant Physiology·Tran Thi Cuc HoaPeter Beyer
Jul 13, 2004·Plant Physiology·Cynthia E GallagherEleanore T Wurtzel
Apr 12, 2005·Plant Physiology·Patrick SchaubPeter Beyer
Dec 16, 2006·Plant Biotechnology Journal·Giovanna GiovinazzoLaura DeGara
Mar 12, 2004·Annual Review of Plant Physiology and Plant Molecular Biology·F. X. Cunningham, E. Gantt
Jan 1, 1997·Annual Review of Microbiology·G A Armstrong
Jul 19, 2011·Journal of Clinical Biochemistry and Nutrition·Takashi MuraguchiYoshihiro Shidoji
Aug 2, 2001·Proceedings of the National Academy of Sciences of the United States of America·P Broun, C Somerville
Mar 4, 2014·Annual Review of Plant Biology·Gemma FarréChangfu Zhu
Jul 11, 2012·Plant Foods for Human Nutrition·Giovanna GiovinazzoAngelo Santino
Jan 13, 2015·Molecular Plant·Nazia NisarBarry J Pogson
Aug 17, 2010·New Biotechnology·M S Swaminathan
Jan 29, 2008·Trends in Biotechnology·Giovanni GiulianoMark A Taylor

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.