Transgenic rice plants expressing the snowdrop lectin gene (gna) exhibit high-level resistance to the whitebacked planthopper (Sogatella furcifera)

TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik
D NagadharaK V Rao

Abstract

Transgenic rice plants, expressing snowdrop lectin [Galanthus nivalis agglutinin (GNA)], obtained by Agrobacterium-mediated genetic transformation, were evaluated for resistance against the insect, the whitebacked planthopper (WBPH). The transgene gna was driven by the phloem-specific, rice-sucrose synthase promoter RSs1, and the bar was driven by the CaMV 35S promoter. In our previous study, the transgenic status of these lines was confirmed by Southern, Northern and Western blot analyses. Both the transgenes, gna and bar, were stably inherited and co-segregated into progenies in T1 to T5 generations. Insect bioassays on transgenic plants revealed the potent entomotoxic effects of GNA on the WBPH. Also, significant decreases were observed in the survival, development and fecundity of the insects fed on transgenic plants. Furthermore, intact GNA was detected in the total proteins of WBPHs fed on these plants. Western blot analysis revealed stable and consistent expression of GNA throughout the growth and development of transgenic plants. Transgenic lines expressing GNA exhibited high-level resistance against the WBPH. As reported earlier, these transgenics also showed substantial resistance against the brown planthopper and gre...Continue Reading

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Citations

Jun 3, 2009·Molecular Genetics and Genomics : MGG·Peter L De HoffAnn M Hirsch
Feb 14, 2006·Transgenic Research·N FerryA M R Gatehouse
Dec 11, 2008·Applied Biochemistry and Biotechnology·Song-hua YeJin-ku Bao
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Nov 30, 2006·Plant Biotechnology Journal·Shavindra Bajaj, Amitabh Mohanty
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May 13, 2006·Trends in Plant Science·Paul ChristouAngharad M R Gatehouse
Dec 20, 2005·Toxicon : Official Journal of the International Society on Toxinology·Jia-Fu JiangKang Chong
Aug 2, 2011·Biotechnology Advances·Varnika BhatiaRamcharan Bhattacharya
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Jan 31, 2015·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Maria Lígia R MacedoCarolina T Oliveira
May 17, 2019·Pest Management Science·Yang LingZhang Weilin
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Mar 29, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Xiaoliang DuanRongqi Liang
Oct 1, 2011·Biotechnology Advances·Parvaiz AhmadF Al-Qurainy

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