Transgenic rice plants harboring an introduced potato proteinase inhibitor II gene are insect resistant

Nature Biotechnology
X DuanR Wu

Abstract

We introduced the potato proteinase inhibitor II (PINII) gene (pin2) into several Japonica rice varieties, and regenerated a large number of transgenic rice plants. Wound-inducible expression of the pin2 gene driven by its own promoter, together with the first intron of the rice actin 1 gene (act1), resulted in high-level accumulation of the PINII protein in the transgenic plants. The introduced pin2 gene was stably inherited in the second, third, and fourth generations, as shown by molecular analyses. Based on data from the molecular analyses, several homozygous transgenic lines were obtained. Bioassay for insect resistance with the fifth-generation transgenic rice plants showed that transgenic rice plants had increased resistance to a major rice insect pest, pink stem borer (Sesamia inferens). Thus, introduction of an insecticidal proteinase inhibitor gene into cereal plants can be used as a general strategy for control of insect pests.

References

Oct 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·T HayakawaS Toriyama
Jan 1, 1991·The Plant Cell·M J Chrispeels, N V Raikhel
Dec 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·R JohnsonC Ryan
Jan 1, 1989·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·C A Ryan
Jan 1, 1993·Progress in Nucleic Acid Research and Molecular Biology·R WuD Xu
Apr 1, 1955·Biochimica Et Biophysica Acta·G W SCHWERT, Y TAKENAKA
Jan 1, 1989·Biotechnology Advances·D BoulterV Hilder
Aug 1, 1989·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·X ZhaoR Wu
Apr 1, 1990·Journal of Chemical Ecology·J L Wolfson, L L Murdock

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Citations

Sep 28, 2005·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Hao ChenQifa Zhang
Oct 12, 2010·Plant Molecular Biology·Jose Luis La PazCarlos M Vicient
May 29, 2003·Journal of Insect Physiology·M A. AndersonR L. Heath
May 29, 2003·Journal of Insect Physiology·C Bolter, M A. Jongsma
May 29, 2003·Journal of Insect Physiology·H S. GatehouseL A. Malone
Nov 7, 2002·Toxicon : Official Journal of the International Society on Toxinology·Célia R Carlini, Maria Fátima Grossi-de-Sá
Sep 21, 2000·Plant Science : an International Journal of Experimental Plant Biology·A K Tyagi, A Mohanty
Nov 14, 2000·Plant Science : an International Journal of Experimental Plant Biology·T Welham, C Domoney
Oct 27, 1998·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·S OverneyC Cloutier
Oct 11, 2003·Biotechnology Advances·C C Giri, G Vijaya Laxmi
Mar 27, 2001·Insect Biochemistry and Molecular Biology·F De LeoL Jouanin
Jul 31, 1998·Insect Biochemistry and Molecular Biology·C GirardL Jouanin
Feb 24, 2001·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·L O BritoM C Silva-Filho
Jun 17, 1998·The Plant Journal : for Cell and Molecular Biology·H KoiwaP M Hasegawa
Dec 21, 2002·European Journal of Biochemistry·Mariateresa VolpicellaJules Beekwilder
Mar 23, 2000·European Journal of Biochemistry·J BeekwilderM Jongsma
Aug 11, 2010·Proceedings of the National Academy of Sciences of the United States of America·K M DunseM A Anderson
Apr 10, 2004·Journal of Experimental Botany·Victoria A JamesPhilippe Vain
Jun 29, 2010·Journal of Experimental Botany·Urte SchlüterDominique Michaud
Oct 3, 2006·Plant Physiology·Purificación LisónVicente Conejero
Jun 25, 2004·Bioscience, Biotechnology, and Biochemistry·Osmundo Brilhante De Oliveira NetoMaria Fátima Grossi-De-Sa
Apr 30, 2003·Proceedings of the National Academy of Sciences of the United States of America·Li LinBaojian Li
Nov 4, 2009·Journal of Enzyme Inhibition and Medicinal Chemistry·Deepa NadarajaBarry Starcher
Jun 23, 2012·Plant Physiology and Biochemistry : PPB·Purushottam R Lomate, Vandana K Hivrale
May 23, 2009·Biotechnology Advances·Giandomenico Corrado, Marianthi Karali
Sep 1, 2009·Environmental Toxicology and Pharmacology·S ZhaoT B Ng
Aug 5, 2008·Plant Physiology and Biochemistry : PPB·Jan SelsMiguel F C De Bolle

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