Transgenic salmon overexpressing growth hormone exhibit decreased myostatin transcript and protein expression

The Journal of Experimental Biology
Steven B RobertsFrederick W Goetz

Abstract

To characterize the role of myostatin (MSTN) in fast growing animals and to examine the relationship between MSTN and growth hormone (GH), MSTN transcript and protein expression were measured in coho salmon overexpressing GH and in wild-type coho salmon. Quantitative real-time RT-PCR and western analysis were used to measure RNA expression of the two salmonid MSTN genes (1 and 2) and levels of MSTN immunoreactive protein (MIP) in developing embryos and adult coho salmon tissues. In transgenic and control coho embryos, MSTN1 and MSTN2 RNA expression were initially observed at about the time of eying, and a 42 kDa MIP was just detected prior to hatching. Expression of the MSTN1 transcript in transgenic salmon was not different from that in wild-type adult coho salmon muscle and brain tissue. However, expression of the MSTN2 transcript was less in white muscle, and greater in red muscle, from transgenic fish compared to wild-type salmon of the same size. Northern analysis revealed that expression of the MSTN2 transcript was less in white muscle from wild-type, age-matched salmon than in transgenic fish. In addition, there was less presumed bioactive MIP in muscle taken from adult transgenic fish compared to controls and evidence o...Continue Reading

References

Nov 14, 1997·Proceedings of the National Academy of Sciences of the United States of America·A C McPherron, S J Lee
Jul 27, 2000·Journal of Cellular Physiology·S KirkJ Bass
Feb 13, 2001·American Journal of Physiology. Endocrinology and Metabolism·W E TaylorN Gonzalez-Cadavid
Feb 13, 2001·Endocrine Reviews·D Le RoithA Butler
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·S J Lee, A C McPherron
Oct 16, 2001·American Journal of Physiology. Cell Physiology·C HuetE Engvall
Oct 19, 2001·European Journal of Biochemistry·T K Østbye Andersen Ø
Nov 10, 2001·American Journal of Physiology. Endocrinology and Metabolism·T J MarcellM R Blackman
Dec 19, 2001·The Journal of Experimental Zoology·F E WeaverD J Coughlin
May 25, 2002·Science·Teresa A ZimmersSe-Jin Lee
Jan 15, 2003·The Journal of Endocrinology·S VianelloL Colombo
Feb 22, 2003·Cell and Tissue Research·Giuseppe RadaelliBruria Funkenstein
Oct 14, 2003·The Journal of Experimental Biology·Cheng XuShao-Jun Du
Nov 7, 2003·The Journal of Clinical Endocrinology and Metabolism·Wei LiuShereen Ezzat
Nov 15, 2003·Molecular and Cellular Endocrinology·Steven B Roberts, Frederick W Goetz
Dec 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Neil M WolfmanSe-Jin Lee

❮ Previous
Next ❯

Citations

Mar 18, 2009·Neuroscience Bulletin·Li HuangXiao-Song Gu
Jul 2, 2008·Endocrine Reviews·Buel D Rodgers, Dilip K Garikipati
May 31, 2014·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Nicholas J GaltPeggy R Biga
Nov 28, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Elisabeth Nadjar-BogerBruria Funkenstein
May 23, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Christian de SantisDean R Jerry
Oct 22, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Bruria FunkensteinAnna Pliatner
Aug 5, 2008·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Iselys DelgadoAlfredo Molina
Nov 8, 2006·Tissue & Cell·B FunkensteinA Rowlerson
Jun 24, 2006·Experimental Cell Research·Dominique Joulia-EkazaCabello Gérard
Jun 7, 2005·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Agnès RevolRoberto Mendoza
Jun 25, 2013·General and Comparative Endocrinology·Eduardo N FuentesBjörn Thrandur Björnsson
Feb 2, 2006·Journal of Muscle Research and Cell Motility·Donglei ZhangXiaosong Gu
May 29, 2009·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Erika F MedeirosTerence M Bradley
Jan 19, 2020·Thyroid : Official Journal of the American Thyroid Association·Cho Rong HanSheue-Yann Cheng

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.