Transgenic soybeans expressing siRNAs specific to a major sperm protein gene suppress Heterodera glycines reproduction

Functional Plant Biology : FPB
Ryan M SteevesHarold N Trick

Abstract

The soybean cyst nematode (SCN), Heterodera glycines, is the major disease-causing agent limiting soybean production in the USA. The current management strategy to reduce yield loss by SCN involves the deployment of resistant soybean cultivars and rotation to non-host crops. Although this management scheme has shown some success, continued yearly yield loss estimates demonstrate the limitations of these techniques. As a result, new control strategies are needed to complement the existing methods. Reported here is a novel method of SCN control that utilises RNA interference (RNAi). Transgenic soybeans were generated following transformation with an RNAi expression vector containing inverted repeats of a cDNA clone of the major sperm protein (MSP) gene from H. glycines. The accumulation of MSP-specific short interfering RNA (siRNA) molecules were detected by northern blot analysis of transgenic soybeans. T0 plants displaying MSP siRNA accumulation were deployed in a bioassay to evaluate the effects of MSP interfering molecules on H. glycines reproduction. Bioassay data has shown up to a 68% reduction in eggs g-1 root tissue, demonstrating that MSPi transgenic plants significantly reduced the reproductive potential of H. glycines....Continue Reading

References

Dec 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·M A Saghai-MaroofR W Allard
Feb 1, 1995·Current Opinion in Cell Biology·T M Roberts, M Stewart
Jul 1, 1996·The Plant Journal : for Cell and Molecular Biology·Y Q AnR B Meagher
Dec 23, 1998·Proceedings of the National Academy of Sciences of the United States of America·M K MontgomeryA Fire
Sep 12, 2000·Current Opinion in Genetics & Development·R H Plasterk, R F Ketting
Feb 7, 2001·Genes & Development·S M ElbashirT Tuschl
Jun 26, 2001·Science·V Vance, H Vaucheret
Jul 12, 2002·Nature·Gregory J Hannon
Aug 17, 2002·Molecular Plant-microbe Interactions : MPMI·P E UrwinHoward J Atkinson
Aug 24, 2002·Science·Thomas A VolpeRobert A Martienssen
Jul 21, 2004·Molecular Cell·Gunter MeisterThomas Tuschl
Jul 31, 2004·Science·Ji-Joon SongLeemor Joshua-Tor
Jul 31, 2004·Science·Jidong LiuGregory J Hannon
Sep 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Tim A RandXiaodong Wang
Jul 27, 2005·Molecular Plant-microbe Interactions : MPMI·M N RossoP Abad
Jul 27, 2005·Molecular Plant-microbe Interactions : MPMI·Qing ChenJohn T Jones
Aug 6, 2005·Proceedings of the National Academy of Sciences of the United States of America·N Baumberger, D C Baulcombe
Oct 12, 2005·International Journal for Parasitology·Catherine J LilleyPeter E Urwin
Nov 1, 2005·Molecular Plant-microbe Interactions : MPMI·Manjula BakhetiaMichael J McPherson
Nov 17, 2005·Genes & Development·Keita MiyoshiMikiko C Siomi
May 9, 2006·Molecular and Biochemical Parasitology·Bindhya Chal YadavKuppuswamy Subramaniam
Jul 1, 1992·Plant Cell Reports·J J FinerM D McMullen

❮ Previous
Next ❯

Citations

Jun 20, 2008·The New Phytologist·Victoria L FullerPeter E Urwin
Apr 30, 2009·Annual Review of Phytopathology·M N RossoP Abad
Sep 1, 2007·Molecular Plant Pathology·Catherine J LilleyPeter E Urwin
Nov 9, 2012·Breeding Science·Megumi Kasai, Akira Kanazawa
Dec 1, 2010·Physiology and Molecular Biology of Plants : an International Journal of Functional Plant Biology·Subodh Kumar Sinha
May 29, 2015·Phytopathology·Isabela Tristan Lourenço-TessuttiMaria Fatima Grossi-de-Sa
Jul 5, 2016·Frontiers in Plant Science·Caspar LangenbachUwe Conrath
Mar 3, 2011·Plant Biotechnology Journal·Jiarui LiHarold N Trick
Nov 27, 2014·Bioengineered·Raja Sekhar NandetyBryce W Falk
Jul 22, 2014·Plant Biotechnology Journal·Aline Koch, Karl-Heinz Kogel
Jan 30, 2015·Frontiers in Microbiology·Tushar K DuttaUma Rao
Nov 6, 2009·Journal of Experimental Botany·Nrupali PatelEric L Davis
Oct 1, 2017·Plant Disease·Richard W SmileyLieven Waeyenberge
Dec 17, 2009·Plant Cell Reports·Jiarui LiHarold N Trick
Jan 7, 2014·PloS One·José Dijair Antonino de Souza JúniorMaria Fatima Grossi-de-Sa
Mar 11, 2015·Journal of Experimental Botany·Alfonso AlbaceteFrancisco Pérez-Alfocea
Jan 24, 2014·Plant Molecular Biology·Shankar R PantVincent P Klink
Jul 6, 2018·Molecular Plant-microbe Interactions : MPMI·Reza Behzadi AminMansour Karimi
Jun 6, 2017·Frontiers in Plant Science·Sagar BanerjeeAnil Sirohi
Jun 24, 2019·TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik·Bin TianHarold N Trick
Sep 29, 2020·Pest Management Science·Juliet OcholaBaldwyn Torto
May 27, 2008·Trends in Biotechnology·Daniel R G Price, John A Gatehouse
Apr 20, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Wayne ParrottMarci Levine

❮ Previous
Next ❯

Methods Mentioned

BETA
transgenic
phosphotransferase
electrophoresis
PCR

Software Mentioned

SAS

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.