Transgenic tomato expressing interleukin-12 has a therapeutic effect in a murine model of progressive pulmonary tuberculosis.

Clinical and Experimental Immunology
A L Elías-LópezR Hernández-Pando

Abstract

Host control of mycobacterial infection, in both human and mouse models, has been shown to be associated with the production of interferon (IFN)-gamma by CD4(+) T cells. Interleukin (IL)-12 is known to be a crucial cytokine in the differentiation of IFN-gamma-producing T helper 1 (Th1) cells. To determine whether continuous administration of IL-12 expressed in transgenic tomato (TT-IL-12) has therapeutic efficacy in a murine model of pulmonary tuberculosis, BALB/c mice were infected with either Mycobacterium tuberculosis H37Rv strain or a multi-drug-resistant clinical isolate (MDR) and treated with a daily oral dose of TT-IL12 crude fruit extracts. For the early H37Rv infection, TT-IL-12 administration was started 1 day before infection and continued for 60 days. In the H37Rv or MDR late infection, treatment was started 60 days after infection and continued for another 60 days. In both phases of infection, TT-IL-12 administration resulted in a reduction of bacterial loads and tissue damage compared with wild-type tomato (non-TT). The Th1 response was increased and the Th2 response was reduced. In the late infection, a long-term treatment with TT-IL-12 was necessary. We demonstrate that TT-IL-12 increases resistance to infection...Continue Reading

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Citations

Jan 21, 2011·Plant Cell Reports·Dawei YuanPaul Christou
May 29, 2010·BMC Research Notes·Carla Sánchez-HernándezBeatriz Gómez-García
Sep 29, 2011·Yi chuan = Hereditas·Ao-Xue Wang, Xiu-Ling Chen
Jan 24, 2014·SpringerPlus·Prati Pal Singh, Amit Goyal
Nov 21, 2009·Clinics in Chest Medicine·Gavin J ChurchyardGraham A Rook
Jul 17, 2013·Biotechnology Journal·Kathleen Hefferon
Aug 3, 2010·Expert Review of Vaccines·Antonio GranellDiego Orzáez
Jul 9, 2021·Frontiers in Cellular and Infection Microbiology·Diego L CostaAlan Sher

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