Transgenic trait deployment using designed nucleases

Plant Biotechnology Journal
Joseph F Petolino, Sandeep Kumar

Abstract

The demand for crops requiring increasingly complex combinations of transgenes poses unique challenges for transgenic trait deployment. Future value-adding traits such as those associated with crop performance are expected to involve multiple transgenes. Random integration of transgenes not only results in unpredictable expression and potential unwanted side effects but stacking multiple, randomly integrated, independently segregating transgenes creates breeding challenges during introgression and product development. Designed nucleases enable the creation of targeted DNA double-strand breaks at specified genomic locations whereby repair can result in targeted transgene integration leading to precise alterations in DNA sequences for plant genome editing, including the targeting of a transgene to a genomic locus that supports high-level and stable transgene expression without interfering with resident gene function. In addition, targeted DNA integration via designed nucleases allows for the addition of transgenes into previously integrated transgenic loci to create stacked products. The currently reported frequencies of independently generated transgenic events obtained with site-specific transgene integration without the aid of...Continue Reading

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Citations

Sep 25, 2016·Planta·Sandeep KumarJoseph F Petolino
Feb 23, 2020·Nucleic Acids Research·Joan Miquel Bernabé-OrtsDiego Orzaez
Aug 24, 2017·Journal of Experimental Botany·Yunbi XuMichael S Olsen
Mar 21, 2019·Frontiers in Bioengineering and Biotechnology·Michael F EckerstorferFriedrich Waßmann
May 21, 2020·Frontiers in Plant Science·Huirong GaoN Doane Chilcoat
Jan 29, 2016·Plant Biotechnology Journal·Joseph F PetolinoHenry Daniell
Nov 10, 2017·Emerging Topics in Life Sciences·Sandeep KumarStephen Novak
Apr 20, 2021·Journal of Plant Physiology·Ravi B Anjanappa, Wilhelm Gruissem

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