Transglutaminases (TGases) form a group of enzymes that have many different substrates and among the most well known are fibrin for Factor XIIIa and the clotting protein in crustaceans. We also found that TGase is an abundant protein in the hematopoietic tissue (Hpt) cells of crayfish and hence we have studied the possible function of this enzyme in hematopoiesis. TGase is one of the most abundant proteins in the Hpt and its mRNA expression as well as enzyme activity is very high in the Hpt cells, lesser in the semi-granular hemocytes and very low in the granular cells. In cultured hematopoietic tissues, high activity was present in cells in the centre of the tissue, whereas cells migrating out of the tissue had very low TGase activity. RNAi experiments using dsRNA for TGase completely knocked down the transcript and as a result the cell morphology was changed and the cells started to spread intensely. If astakine, a cytokine directly involved in hematopoiesis, was added the cells started to spread and adopt a morphology similar to that observed after RNAi of TGase. Astakine had no effect on TGase expression, but after a prolonged incubation for one week with this invertebrate cytokine, TGase activity inside and outside the cel...Continue Reading
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Identification of the major lipoproteins in crayfish hemolymph as proteins involved in immune recognition and clotting
Characterization of a clotting protein, isolated from plasma of the freshwater crayfish Pacifastacus leniusculus
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Biochemical evidence that small proline-rich proteins and trichohyalin function in epithelia by modulation of the biomechanical properties of their cornified cell envelopes.
Transcriptional regulation of cell type-specific expression of the TATA-less A subunit gene for human coagulation factor XIII.
The crayfish plasma clotting protein: a vitellogenin-related protein responsible for clot formation in crustacean blood
Molecular cloning and characterization of a hemolymph clottable protein from tiger shrimp (Penaeus monodon)
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A transglutaminase involved in the coagulation system of the freshwater crayfish, Pacifastacus leniusculus. Tissue localisation and cDNA cloning
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Hemocyte production and maturation in an invertebrate animal; proliferation and gene expression in hematopoietic stem cells of Pacifastacus leniusculus
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Biochemical characterization and cloning of transglutaminases responsible for hemolymph clotting in Penaeus monodon and Marsupenaeus japonicus
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Recent advances in crayfish hematopoietic stem cell culture: a model for studies of hemocyte differentiation and immunity
A novel viral responsive protein is involved in hemocyte homeostasis in the black tiger shrimp, Penaeus monodon.
Invertebrate hematopoiesis: an anterior proliferation center as a link between the hematopoietic tissue and the brain
Comparing the STarT back screening tool's subgroup allocation of individual patients with that of independent clinical experts
The new face of the old molecules: crustin Pm4 and transglutaminase type I serving as rnps down-regulate astakine-mediated hematopoiesis
Reactive oxygen species generated by a heat shock protein (Hsp) inducing product contributes to Hsp70 production and Hsp70-mediated protective immunity in Artemia franciscana against pathogenic vibrios
The known two types of transglutaminases regulate immune and stress responses in white shrimp, Litopenaeus vannamei
Proteomic analysis by iTRAQ in red claw crayfish, Cherax quadricarinatus, hematopoietic tissue cells post white spot syndrome virus infection
Expression of immune-related genes in one phase of embryonic development of freshwater crayfish, Pacifastacus leniusculus
Identification of genes expressed in response to yellow head virus infection in the black tiger shrimp, Penaeus monodon, by suppression subtractive hybridization
Identification and properties of a receptor for the invertebrate cytokine astakine, involved in hematopoiesis
Role of transglutaminase in immune defense against bacterial pathogens via regulation of antimicrobial peptides
The gnotobiotic brine shrimp (Artemia franciscana) model system reveals that the phenolic compound pyrogallol protects against infection through its prooxidant activity
Yellow head virus infection in black tiger shrimp reveals specific interaction with granule-containing hemocytes and crustinPm1 as a responsive protein
Probing the protective mechanism of poly-ß-hydroxybutyrate against vibriosis by using gnotobiotic Artemia franciscana and Vibrio campbellii as host-pathogen model
Expression profiles of astakine-like transcripts in the tarnished plant bug, Lygus lineolaris, exposed to fungal spores of Beauveria bassiana
The second type of transglutaminase regulates immune and stress responses in white shrimp, Litopenaeus vannamei
Dietary supplement of banana (Musa acuminata) peels hot-water extract to enhance the growth, anti-hypothermal stress, immunity and disease resistance of the giant freshwater prawn, Macrobrachium rosenbergii
PDGF/VEGF-Related Receptor Affects Transglutaminase Activity to Control Cell Migration During Crustacean Hematopoiesis
Transglutaminase inhibition stimulates hematopoiesis and reduces aggressive behavior of crayfish, Pacifastacus leniusculus
Over-expression of transglutaminase in the Drosophila eye imaginal disc induces a rough eye phenotype
High salinity induced expression profiling of differentially expressed genes in shrimp (Penaeus monodon)
Phloroglucinol Treatment Induces Transgenerational Epigenetic Inherited Resistance Against Vibrio Infections and Thermal Stress in a Brine Shrimp (Artemia franciscana ) Model
MicroRNA and mRNA interactions coordinate the immune response in non-lethal heat stressed Litopenaeus vannamei against AHPND-causing Vibrio parahaemolyticus.
Reactive Oxygen Species Affect Transglutaminase Activity and Regulate Hematopoiesis in a Crustacean.
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