Transglutaminase-catalysed cross-linking: a potential mechanism for the interaction of fibrinogen, low density lipoprotein and arterial type III procollagen

Thrombosis Research
J M BownessR I Wiebe


Bovine type III [3H]procollagen or its [125I]aminopropeptide were shown by chromatography under dissociating conditions to form very high molecular weight compounds with excess bovine fibrinogen after incubation with purified tissue transglutaminase, though none is formed with other major plasma proteins. Larger compounds of this type formed from fibrinogen or fibrin monomer can be separated by centrifugation and they are insoluble on washing with 1% SDS. Ultracentrifugation showed that a significant fraction of [3H]procollagen III forms a low density complex on incubation with transglutaminase plus excess IDL or LDL, but not HDL. SDS polyacrylamide gel electrophoresis showed that type III collagen [125I]aminopropeptide forms high molecular weight compounds after incubation with transglutaminase plus excess IDL or LDL but not with HDL. It is hypothesized that, in the presence of excessive concentrations of LDL and/or fibrinogen and of tissue transglutaminase, crosslinking reactions of the type demonstrated may interfere with normal injury-repair processes and stimulate the formation of atherosclerotic lesions in arteries.


Jan 1, 1987·Connective Tissue Research·J M BownessK N Dolynchuk
Feb 1, 1988·European Journal of Clinical Investigation·J BonnetH Bricaud
Feb 20, 1986·The New England Journal of Medicine·R Ross
Nov 12, 1987·Nature·M S Brown, J L Goldstein
Jan 1, 1985·Collagen and Related Research·M J Barnes
Dec 8, 1972·Annals of the New York Academy of Sciences·K LakiJ Farrell
Jan 1, 1980·Annual Review of Biochemistry·J E Folk
May 30, 1981·Lancet·E B Smith, E M Staples


Aug 11, 2011·Amino Acids·Shobana ShanmugasundaramMaria V Nurminskaya
Dec 12, 2001·Neurochemistry International·Soo Youl KimPeter M Steinert
Dec 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·S N Murthy, L Lorand
Jun 15, 1997·The Journal of Clinical Investigation·T S JohnsonA Meguid El Nahas
Jul 31, 1990·Biochemical and Biophysical Research Communications·J M Bowness, A H Tarr
Nov 12, 2018·Journal of Thrombosis and Haemostasis : JTH·L G PooleJ P Luyendyk
Jan 28, 2021·International Journal of Molecular Sciences·Mariya E Semkova, J Justin Hsuan

Related Concepts

Bos indicus
Chromatography, Affinity
Cross-Linking Reagents
Chemical Precipitation
Procollagen Type M

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.