Transglutaminase participates in the incorporation of latent TGFbeta into the extracellular matrix of aging articular chondrocytes

Connective Tissue Research
M LeA K Rosenthal

Abstract

TGFbeta1 is a multifunctional peptide growth factor that promotes processes associated with age-related degenerative diseases in articular cartilage. Large quantities of TGFbeta1 are stored in cartilage extracellular matrix (ECM) in a latent form (LTGFbeta1), and yet little is known about the factors that participate in the incorporation of LTGFbeta1 into the highly specialized cartilage ECM. We previously demonstrated high levels of the protein cross-linking enzyme transglutaminase (TGase) in aging articular chondrocytes and showed that this enzyme participated in LTGFbeta1 activation. This work explores the hypothesis that extracellular TGase participates in LTGFbeta1 incorporation into ECM in aging chondrocytes. We studied the effects of TGase inhibitors on TGFbeta1 levels in ECM of old and young porcine articular chondrocytes. TGase inhibitors decreased the quantity of LTGFbeta1 in the ECM in old but not in young chondrocytes to 60-70% of control values (p<.05). Fibronectin, an extracellular TGase competitive substrate, also decreased LTGFbeta1 levels in ECM (p<.01). Levels of activated TGFbeta1 also decreased in the presence of TGase inhibitors, as did levels of latent TGFbeta binding protein 1 in the cell layer. Extracell...Continue Reading

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Citations

Mar 3, 2010·Cell Biology International·Hideki Tatsukawa, Soichi Kojima
Nov 26, 2010·Biochemical and Biophysical Research Communications·Dae-Seok KimSoo-Youl Kim
Jun 13, 2003·Osteoarthritis and Cartilage·A K RosenthalC M Gohr
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