Transglycosylated Starch Modulates the Gut Microbiome and Expression of Genes Related to Lipid Synthesis in Liver and Adipose Tissue of Pigs

Frontiers in Microbiology
Monica A NewmanBarbara U Metzler-Zebeli

Abstract

Dietary inclusion of resistant starches can promote host health through modulation of the gastrointestinal microbiota, short-chain fatty acid (SCFA) profiles, and lipid metabolism. This study investigated the impact of a transglycosylated cornstarch (TGS) on gastric, ileal, cecal, proximal-colonic, and mid-colonic bacterial community profiles and fermentation metabolites using a growing pig model. It additionally evaluated the effect of TGS on the expression of host genes related to glucose and SCFA absorption, incretins, and satiety in the gut as well as host genes related to lipid metabolism in hepatic and adipose tissue. Sixteen growing pigs (4 months of age) were fed either a TGS or control (CON) diet for 11 days. Bacterial profiles were determined via Illumina MiSeq sequencing of the V3-5 region of the 16S rRNA gene, whereas SCFA and gene expression were measured using gas chromatography and reverse transcription-quantitative PCR.Megasphaera, which was increased at all gut sites, began to benefit from TGS feeding in gastric digesta, likely through cross-feeding with other microbes, such asLactobacillus. Shifts in the bacterial profiles from dietary TGS consumption in the cecum, proximal colon, and mid colon were similar. R...Continue Reading

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Citations

Aug 24, 2018·Nutrients·Isabella Pali-SchöllErika Jensen-Jarolim
May 27, 2021·Journal of Animal Science·Barbara U Metzler-ZebeliFrederike Lerch
Jun 22, 2018·Organic Letters·Yong ChenRen Xiang Tan

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Datasets Mentioned

BETA
PRJNA396828

Methods Mentioned

BETA
transglycosylation
scrapings
PCR
RNA Assay
ELISA

Software Mentioned

Greengenes
R Studio
NormFinder
corrplot
BLAST
Primer
QIIME
Microsynth
BestKeeper
ggplot2

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